Hypoxia-induced exosome secretion promotes survival of African-American and Caucasian prostate cancer cells

Scientific Reports
Gati K PanigrahiGagan Deep

Abstract

African American men in the United States have higher mortality due to prostate cancer (PCa) compared to other races. One reason for this disparity is the lack of in-depth understanding of the PCa biology in African Americans. For example, hypoxia in prostate tumor microenvironment is associated with adverse prognosis; still, no hypoxia-related studies have been reported in African Americans. Here, we compared African-American and Caucasian PCa cells for exosome secretion under normoxic (21% O2) and hypoxic (1% O2) conditions. All cell lines showed higher exosome secretion under hypoxia but it was clearly more prominent in African-American PCa cells. Further, under hypoxia, Rab5 (a biomarker for early endosome) was clustered in perinuclear region; and CD63 (a biomarker for exosomes and multivesicular endosomes) showed greater co-localization with actin cytoskeleton especially in African American PCa cells. Importantly, exosome biogenesis inhibitors GW4869 (10-20 µM) or DMA (10-20 µg/ml) significantly decreased cell viability and clonogenicity in PCa cells. Interestingly, we also observed higher level of lactic acid loaded in exosomes secreted under hypoxia. Overall, under chronic hypoxia, PCa cells secrete more exosomes as a su...Continue Reading

References

Mar 17, 2001·Nature Reviews. Molecular Cell Biology·M Zerial, H McBride
Mar 21, 2002·Nature Reviews. Cancer·Adrian L Harris
Apr 30, 2002·Experimental Cell Research·Robert SternBent Formby
Mar 18, 2003·The Journal of Biological Chemistry·Ariel SavinaMaria I Colombo
Jul 16, 2004·Seminars in Radiation Oncology·Stefan Walenta, Wolfgang F Mueller-Klieser
Mar 15, 2006·The Journal of Cell Biology·Amy M BoothStephen J Gould
Mar 28, 2006·International Journal of Radiation Oncology, Biology, Physics·Dawn M CarnellPeter J Hoskin
Jan 27, 2007·Blood·Karin FischerMarina Kreutz
Mar 1, 2008·Science·Katarina TrajkovicMikael Simons
May 20, 2008·Seminars in Cancer Biology·Robert Stern
May 31, 2008·International Journal of Cancer. Journal International Du Cancer·Karl T ButterworthJenny Worthington
Oct 6, 2009·The Journal of Biological Chemistry·Isabella ParoliniStefano Fais
Nov 6, 2009·BJU International·Grant D StewartAntony C P Riddick
Apr 1, 2010·The Journal of Biological Chemistry·Nobuyoshi KosakaTakahiro Ochiya
May 28, 2011·International Journal of Oncology·Kristina GoetzeWolfgang Mueller-Klieser
Oct 12, 2011·International Journal of Radiation Oncology, Biology, Physics·Aruna TurakaBenjamin Movsas
Nov 16, 2011·Cancer Research·Franziska HirschhaeuserWolfgang Mueller-Klieser
Apr 3, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael MilosevicRobert Bristow
Jun 23, 2012·Pharmacological Reviews·Edwin van der PolRienk Nieuwland
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Muller FabbriCarlo M Croce
Sep 25, 2012·BMC Cancer·Hamish W KingJonathan M Gleadle
Dec 14, 2012·World Journal of Surgical Oncology·Youcheng DingZhenggang Zhu
Feb 7, 2013·CA: a Cancer Journal for Clinicians·Carol DeSantisAhmedin Jemal
Mar 23, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Christoph Kahlert, Raghu Kalluri
Apr 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Paulina KucharzewskaMattias Belting
Jun 5, 2013·The Journal of Pathology·Stephen Yiu Chuen ChoiYuzhuo Wang
Oct 18, 2013·The Journal of Biological Chemistry·Hiroko TadokoroJunko H Ohyashiki
Dec 3, 2013·Cell Reports·Daisuke HoshinoAlissa M Weaver
Aug 1, 2014·International Journal of Biological Sciences·Shahriar KoochekpourKristopher Attwood
May 27, 2015·Clinical Genitourinary Cancer·Avelino FragaRui Medeiros
Feb 26, 2016·CA: a Cancer Journal for Clinicians·Carol E DeSantisAhmedin Jemal

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Citations

Nov 15, 2019·Current Pharmaceutical Design·Biancamaria LongoniRoberto Maggio
Mar 21, 2020·IUBMB Life·Sajad YaghoubiNader Bagheri
Sep 13, 2019·Journal of Cellular Physiology·Aman Sharma, Abiya Johnson
Jul 2, 2020·Cancers·Dilip NarayananDennis Özcelik
Jan 14, 2020·British Journal of Cancer·Amaelle OtandaultAlain R Thierry
Jun 18, 2020·Cells·Patrick Santos, Fausto Almeida
Oct 18, 2018·Frontiers in Physiology·Daniela CesselliAntonio Paolo Beltrami
Nov 9, 2019·International Journal of Molecular Sciences·Tünde SzatmáriKatalin Lumniczky
Mar 13, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Mirza S BaigKishore K Wary
Jun 7, 2020·Journal of Hematology & Oncology·Wenyu WangYong Sang Song
Feb 1, 2020·Journal of Extracellular Vesicles·Mariadelva Catalano, Lorraine O'Driscoll
Feb 23, 2019·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Stanley E HookerRick A Kittles
Dec 5, 2020·Trends in Cancer·Giulia FuresiLorenz C Hofbauer
Dec 12, 2020·Journal of Translational Medicine·Reza JafariJafar Rezaie
May 23, 2020·Cancer Letters·Mahendran ChinnappanRajagopal Ramesh
May 31, 2020·Cancer Letters·Ashish Kumar, Gagan Deep
May 1, 2021·Journal of Personalized Medicine·Katalin BalázsKatalin Lumniczky

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Methods Mentioned

BETA
electron microscopy
transmission electron microscopy
GTPases
confocal microscopy
MDA
Protein Assay
Assay

Software Mentioned

Graphpad Prism

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