Hypoxia-induced feedback of HIF-1α and lncRNA-CF129 contributes to pancreatic cancer progression through stabilization of p53 protein

Theranostics
Mingliang LiuGang Zhao

Abstract

Rationale: Emerging evidences have highlighted the critical roles of lncRNAs in human cancer development. The work sought to assess the biological role and potential underlying mechanisms of lncRNA-CF129 (CF129) which is significantly reduced in pancreatic cancer (PC). Methods: CF129 expression and its association with multiple clinicopathologic characteristics in PC specimens were analyzed. The role of CF129 both in vitro and in vivo was assessed, with RNA pull-down and immunoprecipitation assays being performed to detect the interaction between CF129 and p53 and E3 ligase MKRN1. Chromatin immunoprecipitation and luciferase assays were utilized to identify the interaction between p53 and FOXC2 promoter, HIF-1α/HDAC1 complex and CF129 promoter, FOXC2 and HIF-1α promoter, respectively. Results: CF129 levels were markedly lower in PC compared with paired non-tumor adjacent tissues. Low CF129 expression predicted short overall survival in PC patients. CF129 inhibited invasion and metastasis of PC cells in a FOXC2-dependent manner. In addition, CF129 regulates FOXC2 transcription through association with mutant p53. CF129 directly binds to p53 and E3 ligase MKRN1, and such an interaction leading to p53 protein ubiquitination and de...Continue Reading

Citations

Jun 10, 2020·Cancer Cell International·Purong ZhangQing Lu
Jun 27, 2020·Cancer Cell International·Xiao JinZheng Liu
May 7, 2020·Journal of Biomedical Science·Tse-Chun KuoJing-Wen Shih
Oct 30, 2020·Cells·Claudia Geismann, Alexander Arlt
Jan 14, 2021·Journal of Hematology & Oncology·Jinxin TaoYupei Zhao
Feb 2, 2021·Frontiers in Oncology·Yuming XuZhengjun Shang
Mar 31, 2020·Nanomedicine : Nanotechnology, Biology, and Medicine·Susann PiehlerIngrid Hilger
Mar 7, 2021·International Journal of Molecular Sciences·Maria Magdalena BarrecaAlice Conigliaro
May 27, 2021·Transcription·K T Ramya DeviSanjana Prakash Hemdev
Jul 12, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Li YouKamil Kuca
Aug 4, 2021·Cancer Metastasis Reviews·Nianhong ChenPing Shi
Sep 15, 2020·Seminars in Cancer Biology·Song ChenLianxin Liu

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Datasets Mentioned

BETA
GDS4102

Methods Mentioned

BETA
ubiquitination
transfection
xenograft
pulldown
immunoprecipitation
ChIP
Co-IP
RIP
RNA-pull down
ELISA

Software Mentioned

AnnoLnc
GraphPad Prism
SPSS
Jaspar
catRAPID

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