Hypoxia induced ferritin light chain (FTL) promoted epithelia mesenchymal transition and chemoresistance of glioma.

Journal of Experimental & Clinical Cancer Research : CR
Junhui LiuQianxue Chen

Abstract

Hypoxia, a fundamental characteristic of glioma, is considered to promote tumor malignancy by inducing process of epithelial mesenchymal transition (EMT). Ferritin Light Chain (FTL) is one of the iron metabolism regulators and is overexpressed in glioma. However, relationship between hypoxia and FTL expression and its role in regulating EMT remains unclear. Immunohistochemistry (IHC), western blot and public datasets were used to evaluate FTL level in glioma. Wound healing, transwell assays, CCK8, annexin V staining assay were used to measure migration, invasion, proliferation and apoptosis of glioma cells in vitro. Interaction between HIF1A and FTL was assessed by luciferase reporter and Chromatin immunoprecipitation (ChIP) assays. Subcutaneous xenograft model was established to investigate in vivo growth. FTL expression was enriched in high grade glioma (HGG) and its expression significantly associated with IDH1/2 wildtype and unfavorable prognosis of glioma patients. FTL expression positively correlated with HIF1A in glioma tissues and obviously increased in U87 and U251 cells under hypoxia in a time-dependent manner. Mechanistically, HIF-1α regulates FTL expression by directly binding to HRE-3 in FTL promoter region. Furthe...Continue Reading

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Citations

Jan 12, 2021·Journal of Experimental & Clinical Cancer Research : CR·Muhlis AkmanChiara Riganti
Apr 25, 2021·Cellular and Molecular Neurobiology·Renxuan HuangLiankun Sun
Jul 3, 2021·Frontiers in Cell and Developmental Biology·Runze QiuHongwei Fan
Aug 28, 2021·International Journal of Molecular Sciences·Kim I AdameykoYulia V Lyupina

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Methods Mentioned

BETA
RNAseq
PCR
protein assay
FACS
xenografts
Assay
immunoprecipitation
ChIP
transfection
xenograft

Software Mentioned

GraphPad
UCSC
ImageJ
SPSS
Image J
GraphPad Prism
Xena

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