Hypoxia-induced hyaluronan synthesis by articular chondrocytes: the role of nitric oxide

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
K HashimotoC Hamanishi

Abstract

Articular cartilage is an avascular tissue in which chondrocytes are exposed to hypoxic conditions. We previously demonstrated that reactive oxygen species (ROS) induced apoptosis of chondrocytes. We also demonstrated that nitric oxide (NO) was induced when chondrocytes were exposed to hypoxia and that NO inhibited the ROS-induced apoptosis. Hyaluronan (HA) is a high molecular weight glycosaminoglycan whose antioxidative effects have been reported. The purpose of the present study was to determine whether HA synthesis was induced in chondrocytes exposed to hypoxia, and, if so, whether the hypoxia-induced HA synthesis is regulated by NO. Bovine articular chondrocytes were used in this study. Levels of HA were determined by the sandwich enzyme-binding assay. Expression of HA synthase (HAS) was determined with reverse transcription-polymerase chain reaction. The production of NO was examined using the Griess reaction. We also determined inducible nitric oxide synthase (iNOS) enzyme synthesis using the histochemistry and Western blot analysis. Chondrocytes cultured under hypoxic conditions exhibited enhanced HA synthesis. When the NO inhibitors, L-NMMA and L-NAME, were added, the hypoxia-enhanced HA levels in the culture medium wer...Continue Reading

Citations

Sep 30, 2010·Respirology : Official Journal of the Asian Pacific Society of Respirology·Pei-ming HuangCharles A Hales
Sep 9, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Metin AytekinRaed A Dweik
Oct 13, 2009·BMC Molecular Biology·Casper Bindzus FoldagerMartin Lind
Jul 31, 2008·PloS One·Gidon OfekKyriacos A Athanasiou
Dec 9, 2014·Case Reports in Dermatological Medicine·Alexandra Caitlin Perel-Winkler, Chris T Derk
Jul 21, 2009·Journal of Cutaneous Pathology·Rupa PugashettiTimothy Berger
May 16, 2014·International Journal of Cancer. Journal International Du Cancer·Chi ZhangYing Xu
Jul 17, 2010·Osteoarthritis and Cartilage·B FermorB O Diekman
Jan 27, 2015·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·R DasH Jahr
Sep 11, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Peeter KarihtalaVeli-Matti Kosma
Jun 20, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Clarissa R CoveneyAngus K T Wann
Feb 28, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Rainer J EgliMichael Leunig
Aug 27, 2017·American Journal of Physiology. Heart and Circulatory Physiology·Dorothy AmofaKatherine E Yutzey

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Christian H CoyleConstance R Chu
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Siriporn PeansukmaneeAli Mobasheri
© 2022 Meta ULC. All rights reserved