PMID: 8944720Nov 1, 1996Paper

Hypoxia-induced pulmonary arterial contraction appears to be dependent on myosin light chain phosphorylation

The American Journal of Physiology
Y ZhaoC S Packer

Abstract

The signal transduction pathway of hypoxic pulmonary arterial contraction has not been elucidated. Phosphorylation of the 20-kDa myosin light chain (MLC20) is thought to be essential for vascular muscle contraction. However, there are reports that smooth muscle will contract in response to nonphysiological stimuli such as phorbol esters without the involvement of MLC20 phosphorylation. The purpose of this study was to determine if hypoxia-induced pulmonary arterial contraction is dependent on MLC20 phosphorylation. Isolated rat pulmonary and carotid (for comparative purposes) arterial strips were contracted with 80 mM KCl to establish maximum active tension in response to membrane depolarization. The strips were then stimulated with one of the following: 30 mM KCl, 1 microM phenylephrine, 0.01 microM angiotensin II, 1 microM phorbol 12-myristate 13-acetate (PMA), or hypoxia (95% N2-5% CO2). In some experiments ML-9, a myosin light chain kinase inhibitor, or calphostin C, a protein kinase C (PKC) inhibitor, was introduced into the bath before hypoxia. Isometric tension was recorded as a function of time. Muscle strips were freeze-clamped (liquid N2) at various time points during the course of responses to the various stimuli. ML...Continue Reading

Citations

Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Andrea OlschewskiE Kenneth Weir
Jun 19, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jian WangJ T Sylvester
Jun 18, 2005·Journal of Applied Physiology·Jennifer L LosapioAlan H Stephenson
Jun 15, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Letitia WeigandJ T Sylvester
Apr 26, 2003·American Journal of Respiratory Cell and Molecular Biology·Zhiqian WangRodney A Rhoades
Dec 14, 2004·Journal of Applied Physiology·Gregory B Waypa, Paul T Schumacker
Feb 10, 2004·Journal of Applied Physiology·Ganesh K Kumar, Jon B Klein
Feb 3, 2012·Physiological Reviews·J T SylvesterJeremy P T Ward
Sep 20, 2000·American Journal of Physiology. Heart and Circulatory Physiology·N J PelaezC S Packer
Sep 14, 2006·Journal of Applied Physiology·A Mark Evans, Constancio Gonzalez

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