Hypoxia-induced small extracellular vesicle proteins regulate proinflammatory cytokines and systemic blood pressure in pregnant rats.

Clinical Science
Suchismita DuttaCarlos Salomon

Abstract

Small extracellular vesicles (sEVs) released from the extravillous trophoblast (EVT) are known to regulate uterine spiral artery remodeling during early pregnancy. The bioactivity and release of these sEVs differ under differing oxygen tensions and in aberrant pregnancy conditions. Whether the placental cell-derived sEVs released from the hypoxic placenta contribute to the pathophysiology of preeclampsia is not known. We hypothesize that, in response to low oxygen tension, the EVT packages a specific set of proteins in sEVs and that these released sEVs interact with endothelial cells to induce inflammation and increase maternal systemic blood pressure. Using a quantitative MS/MS approach, we identified 507 differentially abundant proteins within sEVs isolated from HTR-8/SVneo cells (a commonly used EVT model) cultured at 1% (hypoxia) compared with 8% (normoxia) oxygen. Among these differentially abundant proteins, 206 were up-regulated and 301 were down-regulated (P < 0.05), and they were mainly implicated in inflammation-related pathways. In vitro incubation of hypoxic sEVs with endothelial cells, significantly increased (P < 0.05) the release of GM-CSF, IL-6, IL-8, and VEGF, when compared with control (i.e. cells without sEVs...Continue Reading

References

Apr 13, 1990·Science·S KovatsR DeMars
Mar 1, 1997·American Journal of Reproductive Immunology : AJRI·K P Conrad, D F Benyo
Aug 3, 2002·Nature Reviews. Immunology·Clotilde ThérySebastian Amigorena
Apr 18, 2003·Journal of the Society for Gynecologic Investigation·Christy M IslerJoey P Granger
Jul 11, 2006·BJOG : an International Journal of Obstetrics and Gynaecology·P M Catalano, H M Ehrenberg
Oct 16, 2008·The Journal of Clinical Endocrinology and Metabolism·Charles C ChungJames E Hixson
Jul 3, 2009·Biology of Reproduction·Natalie J HannanLois A Salamonsen
Mar 23, 2012·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Mami IshikuroYutaka Imai
Jan 23, 2013·Hypertension·Vijayakumar ChinnathambiKunju Sathishkumar
Feb 5, 2013·Biochemical and Biophysical Research Communications·Ying ChenRichard Leach
Aug 12, 2014·Journal of Translational Medicine·Suchismita SarkerCarlos Salomon
Aug 27, 2014·Frontiers in Pharmacology·Carlos SalomonGregory E Rice
Aug 5, 2015·The Journal of Clinical Endocrinology and Metabolism·Gregory E RiceCarlos Salomon
Oct 3, 2015·American Journal of Obstetrics and Gynecology·Murray D MitchellCarlos Salomon
Mar 24, 2016·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·A OzmenE T Korgun
Nov 25, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Duo ZhangYang Jin
Feb 6, 2017·Placenta·Wassim Abou-KheirGeorges Daoud
Mar 25, 2017·Molecular & Cellular Proteomics : MCP·Stefan BjelosevicVera Ignjatovic
Apr 12, 2017·The Journal of Clinical Endocrinology and Metabolism·Hong-Hai ZhangDong-Bao Chen
May 14, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Eva-Maria Krämer-Albers
May 23, 2017·The Journal of Clinical Endocrinology and Metabolism·Carlos SalomonGregory E Rice
Sep 25, 2017·Current Hypertension Reports·George OsolMaurizio Mandalà
Dec 8, 2017·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sarah A MarshallLaura J Parry
Apr 7, 2018·Placenta·Mirthe H SchootsJan-Luuk Hillebrands
Jun 10, 2018·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Emre ZaferHasan Yuksel
Jun 30, 2018·Human Reproduction·Joanna L JamesAlys R Clark
Jul 20, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhi-Hui XiongHong-Ling Geng
Oct 26, 2018·Endocrinology·Ramkumar MenonUNKNOWN Garbhini Study Team
Feb 3, 2019·The Journal of Steroid Biochemistry and Molecular Biology·Linnea BärebringHanna Augustin

❮ Previous
Next ❯

Citations

Nov 5, 2020·The Journal of Obstetrics and Gynaecology Research·Xiaoqin WangHe Fei
Feb 4, 2021·American Journal of Reproductive Immunology : AJRI·Shu Li, Yan-Wei Hu
Mar 30, 2021·Biology of Reproduction·Zheng WangJinjun Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.