PMID: 7539223May 1, 1995Paper

Hypoxia induces capillary network formation in cultured bovine pulmonary microvessel endothelial cells

The American Journal of Physiology
P G PhillipsA Narendran

Abstract

The development of new vessels (angiogenesis) is essential to wound healing. The center of a wound space is hypoxic, a condition that has been shown to stimulate angiogenesis in animal models of coronary artery occlusion. Because the mechanisms involved in this complex process are difficult to study in situ, an in vitro model would provide a useful complement to in vivo studies. This laboratory has developed and characterized calf pulmonary microvessel endothelial cell (PMVEC) cultures and an in vitro model system of angiogenesis using collagen three-dimensional gels that permit migration of cells into vessel networks. This system was used to study the direct effect of normoxia (20% O2) or hypoxia (5% O2) on PMVEC ability to undergo angiogenesis in vitro. Major changes leading to formation of capillary-like networks occurred during the first 3 days of hypoxic exposure only and included restructuring of actin filament networks, focal changes in distribution of basic fibroblast growth factor, and orientation and migration of cell tracts into a collagen gel matrix to form vessel networks.

Citations

Mar 4, 2011·American Journal of Physiology. Cell Physiology·Donghoon YoonJosef T Prchal
Oct 29, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·E PapakonstantinouM Roth
Apr 9, 2020·American Journal of Physiology. Cell Physiology·Chi ZhouJing Zheng
Jan 24, 2003·American Journal of Respiratory Cell and Molecular Biology·Shama AhmadCarl W White
Mar 19, 2009·Tissue Engineering. Part a·Craig K Griffith, Steven C George
Jun 19, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·P G PhillipsW L Milonovich

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