Hypoxia induces complex I inhibition and ultrastructural damage by increasing mitochondrial nitric oxide in developing CNS

The European Journal of Neuroscience
Sebastián GiustiSara Fiszer de Plazas

Abstract

NO-mediated toxicity contributes to neuronal damage after hypoxia; however, the molecular mechanisms involved are still a matter of controversy. Since mitochondria play a key role in signalling neuronal death, we aimed to determine the role of nitrative stress in hypoxia-induced mitochondrial damage. Therefore, we analysed the biochemical and ultrastructural impairment of these organelles in the optic lobe of chick embryos after in vivo hypoxia-reoxygenation. Also, we studied the NO-dependence of damage and examined modulation of mitochondrial nitric oxide synthase (mtNOS) after the hypoxic event. A transient but substantial increase in mtNOS content and activity was observed at 0-2 h posthypoxia, resulting in accumulation of nitrated mitochondrial proteins measured by immunoblotting. However, no variations in nNOS content were observed in the homogenates, suggesting an increased translocation to mitochondria and not a general de novo synthesis. In parallel with mtNOS kinetics, mitochondria exhibited prolonged inhibition of maximal complex I activity and ultrastructural phenotypes associated with swelling, namely, fading of cristae, intracristal dilations and membrane disruption. Administration of the selective nNOS inhibitor 7...Continue Reading

References

May 15, 1976·The Biochemical Journal·A BoverisA O Stoppani
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D S Bredt, S H Snyder
Nov 1, 1980·The Biochemical Journal·J F Turrens, A Boveris
Jul 17, 1995·Biochimica Et Biophysica Acta·M Zoratti, I Szabò
Feb 1, 1993·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·D RibattiM Bertossi
Jun 6, 1998·The Journal of Biological Chemistry·C GiuliviA Boveris
Jun 6, 1998·The Journal of Biological Chemistry·A Tatoyan, C Giulivi
Jan 12, 2000·Free Radical Research·S P GossB Kalyanaraman
Dec 13, 2000·Brain Research. Developmental Brain Research·D J Rodríguez GilS Fiszer de Plazas
Jan 13, 2001·Brain Research. Developmental Brain Research·M Puka-SundvallH Hagberg
Mar 10, 2001·Mental Retardation and Developmental Disabilities Research Reviews·J J Volpe
Nov 16, 2001·American Journal of Physiology. Heart and Circulatory Physiology·M C CarrerasJ J Poderoso
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·A J KanaiJ Peterson
Aug 3, 2002·The Journal of Biological Chemistry·Sarah Liv ElferingCecilia Giulivi
Dec 17, 2002·Methods in Enzymology·María Cecilia CarrerasJuan José Poderoso
Feb 5, 2003·Free Radical Biology & Medicine·Cecilia Giulivi
Mar 1, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Jorge Guillermo PeraltaJuan José Poderoso
Mar 20, 2003·Molecular & Cellular Proteomics : MCP·Joe CarrollJohn E Walker
Apr 12, 2003·Redox Report : Communications in Free Radical Research·Itaro HattoriJunji Yodoi
Dec 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lorenz SchildWolfgang Augustin
Jan 9, 2004·IUBMB Life·Virginia HaynesCecilia Giulivi
Mar 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Rafael Radi
Apr 16, 2004·The Journal of Biological Chemistry·Thomas KoeckKulwant S Aulak
Oct 16, 2004·Neuroreport·Inês M AraújoCaetana M Carvalho
Feb 12, 2005·Journal of Applied Physiology·Tamara ZaobornyjAlberto Boveris
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Taku SugawaraPak H Chan
Jun 24, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Joseph SolienCecilia Giulivi
Oct 27, 2005·Journal of Biochemistry and Molecular Biology·Seok Joon WonByoung Joo Gwag
Jul 27, 2006·Neurochemical Research·Marina VacottoSara Fiszer de Plazas
Aug 4, 2006·American Journal of Physiology. Cell Physiology·Cecilia GiuliviChristopher Eric Cooper

❮ Previous
Next ❯

Citations

Apr 2, 2010·American Journal of Physiology. Cell Physiology·Robert M DouglasSameh S Ali
Nov 26, 2009·The International Journal of Biochemistry & Cell Biology·Petr JezekRodrigue Rossignol
May 12, 2009·Biochemical Pharmacology·Hui WangLingyun Wu
May 23, 2009·Neurochemical Research·Sebastián GiustiSara Fiszer de Plazas
Sep 29, 2011·Journal of Neuroscience Research·Sebastián Giusti, Sara Fiszer de Plazas
Mar 6, 2019·The Journal of Experimental Biology·Jules B L DevauxGillian M C Renshaw
Jan 23, 2009·Archives of Biochemistry and Biophysics·Juan J Poderoso

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.