PMID: 10226931May 5, 1999Paper

Hypoxia induces differential changes of dopamine metabolism in mature and immature mesencephalic and diencephalic cell cultures

Journal of Neural Transmission
J GaoK Andersson

Abstract

Perinatal hypoxia is known as a high risk factor for the development of long-lasting abnormalities in dopaminergic system. The early developmental alterations of dopamine (DA) metabolism induced by hypoxia could contribute to these abnormalities. To understand the hypoxia-induced changes of intra- and extracellular dopamine levels and its main metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), in immature dopaminergic neurons, we compared these changes in rat mesencephalic and diencephalic cell cultures on day in vitro (DIV) 2 (immature cells), DIV 8 and DIV 13 (mature cells). Cell cultures were exposed to an oxygen-free gas mixture in a Billups chamber for 2-4 hours. Mature cell cultures responded to hypoxia with an increase of DA levels in the cells and in the medium during the first 45 min (by an average of 57 and 114% respectively). Thereafter, DA levels decreased, and returned to the baseline within the next 30 min. The cellular DA levels continued to decrease up to 15% of the baseline during 255 min hypoxia whereas the extracellular DA content stabilized at the prehypoxic levels. Immature cell cultures (DIV 2) in contrast to mature ones, were unable to maintain normal extracellular DA levels ...Continue Reading

Citations

Mar 20, 2002·Current Neurology and Neuroscience Reports·M Scher
Jun 1, 2005·Hearing Research·Johann GrossBirgit Mazurek
Sep 19, 2003·Hearing Research·Johann GrossT Kietzmann
Mar 31, 2011·Hearing Research·Birgit MazurekJohann Gross
Jun 28, 2002·The Tohoku Journal of Experimental Medicine·Ibrahim GünerTülin Oruç
Apr 1, 2014·Experimental Neurology·Kara M Smith, Nabila Dahodwala

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