Hypoxia induces nitric oxide synthase in rheumatoid synoviocytes: consequences on NADPH oxidase regulation

Free Radical Research
C Chenevier-GobeauxD Borderie

Abstract

We investigated the effects of hypoxia on inducible NO synthase (iNOS) activity and expression in rheumatoid arthritis (RA) synoviocytes. We further studied the relationship between nitrosative stress and NADPH oxidase (NOX) in such conditions. Human cultured synoviocytes were treated for 24 hours with IL-1β, TNF-α or neither, and submitted to hypoxia or normoxia for the last 6 hours. Nitrite production and iNOS expression were increased under hypoxia conditions in RA cells in comparison to normoxia. Hypoxia did not potentate the basal and cytokine-induced superoxide productions, while NOXs' subunit expression and p47-phox phosphorylation were increased. Nitrosylation of NOXs and p47-phox was not raised under hypoxia conditions. Finally, peroxynitrite production was significantly increased under hypoxia conditions, in comparison to normoxia. Our results provide evidence for upregulation of iNOS and NOX activities in RA synoviocytes under hypoxia conditions, associated to an increased peroxynitrite production. Synovial cell metabolism under hypoxia conditions might be different from that in normoxia.

References

Aug 1, 1997·Arthritis and Rheumatism·P HilliquinO G Ekindjian
Feb 19, 1998·Archives of Biochemistry and Biophysics·O InanamiB M Babior
Jun 26, 1998·Free Radical Biology & Medicine·D Jang, G A Murrell
Aug 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andreas Von Knethen, Bernhard Brüne
May 3, 2003·Trends in Molecular Medicine·Matthew W FosterJonathan S Stamler
Nov 13, 2004·Arthritis Research & Therapy·Carol A Hitchon, Hani S El-Gabalawy
Jul 23, 2005·Biochemical and Biophysical Research Communications·Nicole SchneiderDidier A Serteyn
Nov 24, 2006·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·C Chenevier-GobeauxD Borderie
Jun 15, 2007·Immunology Letters·György NagyAndrew P Cope
Feb 12, 2008·The Journal of Hand Surgery·Branavan SivakumarNorbert Kang
May 12, 2009·Arthritis Research & Therapy·Mohammed A AkhavaniEwa M Paleolog
Aug 27, 2009·Annals of the Rheumatic Diseases·Monika BinieckaJacintha N O'Sullivan
Nov 10, 2009·Nature Medicine·Stephanie LefèvreElena Neumann
May 5, 2010·Annals of the Rheumatic Diseases·C T NgD J Veale
Sep 18, 2010·Arthritis and Rheumatism·Manuel J Del ReyJosé L Pablos
Jan 13, 2011·Arthritis and Rheumatism·Aisling KennedyDouglas J Veale
Sep 8, 2011·Immunology Letters·Jean-Baptiste DaudinCarole Nicco

❮ Previous
Next ❯

Citations

Aug 27, 2014·Arthritis & Rheumatology·Monika BinieckaUrsula Fearon
Jan 20, 2017·Experimental and Therapeutic Medicine·Shu-Hua JiangZhi-Juan Sun

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.