PMID: 8945922Nov 1, 1996Paper

Hypoxia induces PECAM-1 phosphorylation and transendothelial migration of monocytes

The American Journal of Physiology
Vijay K KalraV Rattan

Abstract

Polymorphonuclear neutrophils (PMN) adhere to the vascular endothelium under hypoxic conditions, causing microvascular injury. The molecular mechanism of hypoxia-induced adhesion of PMN to and diapedesis through the vascular endothelium is poorly understood. We examined the effects of hypoxia on the transendothelial migration of monocytes. Exposure of human umbilical vein endothelial cells (HUVEC) cultured in Transwell chambers under low oxygen tension (3% O2 compared with 21% O2) resulted in an increased rate of migration of both monocyte-like HL-60 cells and human peripheral blood monocytes. Migration was inhibited by addition of an antibody to platelet endothelial cell adhesion molecule-1 (PECAM-1), a protein kinase C (PKC) inhibitor, or a platelet-activating factor (PAF)-receptor antagonist. In HUVEC, hypoxic conditions (1, 3, 5, and 14% O2) increased the phosphorylation of PECAM-1. The extent of phosphorylation of PECAM-1 was inversely related to the concentration of oxygen to which HUVEC were exposed. Hypoxia-induced phosphorylation of PECAM-1 was inhibited by either a PKC inhibitor or a PAF-receptor antagonist, indicating the involvement of hypoxia-induced release of PAF in both PKC activation and the concomitant phospho...Continue Reading

Citations

Feb 10, 2004·Journal of Applied Physiology·Ganesh K Kumar, Jon B Klein
Oct 9, 2007·Annual Review of Pharmacology and Toxicology·Eric ChurchillDaria Mochly-Rosen
Oct 4, 2000·Physiological Reviews·G MontrucchioG Camussi
Aug 15, 2002·American Journal of Physiology. Cell Physiology·Ranjit GiriVijay K Kalra
Nov 18, 2000·American Journal of Physiology. Cell Physiology·R GiriV K Kalra

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