Hypoxia induces resistance to 5-fluorouracil in oral cancer cells via G(1) phase cell cycle arrest

Oral Oncology
Sayaka YoshibaSatoru Shintani

Abstract

Malignant tumors are exposed to various levels of hypoxic condition in vivo. It has been known that tumor cells under hypoxia are resistant to chemotherapies. To clarify the mechanism of the hypoxia-induced chemoresistance, we evaluated the effects of hypoxia on the resistance of oral squamous cell carcinoma (OSCC) cell lines to 5-fluorouracil (5-FU). OSCC cells were divided to two groups by the proliferation activity under hypoxic condition; hypoxia-resistant (HR) and hypoxia-sensitive (HS) cells. Growth of HS cells were inhibited by hypoxia and introduced to G(1) arrest in cell cycle. 5-FU effect on HS cell viability was markedly reduced in hypoxic condition without an induction of chemoresistant related protein, P-glycoprotein. However, proliferation, cell cycle, and 5-FU sensitivity of HR cells were not affected by hypoxia. Hypoxia-inducible factor (HIF)-1alpha was induced by hypoxia in all OSCC cell lines, but diminished in HS cells within 48h. Expression of p21 and p27 was strongly augmented and CyclinD expression was reduced by hypoxia in HS cells. However, the expression of these proteins was constitutive in HR cells during 48h hypoxic culture. Phosphorylation of mammalian target of rapamycin (mTOR) was reduced by hypox...Continue Reading

References

Jun 1, 1994·Cancer Metastasis Reviews·B A Teicher
Mar 31, 2000·Molecular Medicine Today·J M Brown
Jan 20, 2004·Trends in Biochemical Sciences·David Carling
Jan 20, 2004·Trends in Biochemical Sciences·Yong LiKun-Liang Guan
Apr 23, 2004·Trends in Cell Biology·Duojia PanXinsheng Gao
Jun 2, 2004·Nature Reviews. Cancer·J Martin Brown, William R Wilson
Sep 25, 2004·International Journal of Cancer. Journal International Du Cancer·Maria WartenbergHeinrich Sauer
Mar 23, 2005·Biochemical and Biophysical Research Communications·Y HamanakaM Inoue
Jun 14, 2005·Head & Neck·H L JanssenA C Begg
Jul 1, 2005·Molecular and Cellular Biology·Avi SoferLeif W Ellisen
Feb 18, 2006·Molecular Cell·Liping LiuM Celeste Simon
Aug 3, 2006·Nature Reviews. Drug Discovery·Sandrine FaivreEric Raymond
Oct 17, 2006·Oncogene·J B Easton, P J Houghton
Oct 27, 2006·International Journal of Cancer. Journal International Du Cancer·Eri SasabeTokio Osaki

❮ Previous
Next ❯

Citations

Oct 17, 2014·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yan-Mei ZouHua Xiong
Feb 16, 2015·Pathology Oncology Research : POR·Tomofumi NaruseMasahiro Umeda
May 23, 2015·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Helena DoktorovaTomas Eckschlager
Apr 11, 2013·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Cheng-Qin WangWen-Juan Yu
Oct 1, 2011·Cancer Letters·Mario Pérez-SayánsAbel García-García
Dec 18, 2013·Photochemistry and Photobiology·Megan A Mackey, Mostafa A El-Sayed
Oct 3, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Da-Wei LiLiang Zhou
Jun 24, 2017·Journal of Experimental & Clinical Cancer Research : CR·Xiaodong FengFuxiang Chen
Oct 30, 2020·International Journal of Molecular Sciences·Polina V ShnaiderVictoria O Shender
Jul 18, 2020·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Nuray ErinThomas Efferth
Apr 2, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kathryn M CamachoSamir Mitragotri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

© 2022 Meta ULC. All rights reserved