Hypoxia Inducible Factor 1α Plays a Key Role in Remote Ischemic Preconditioning Against Stroke by Modulating Inflammatory Responses in Rats

Journal of the American Heart Association
Jian YangHeng Zhao

Abstract

Limb remote ischemic preconditioning (RIPC) protects against brain injury induced by stroke, but the underlying protective mechanisms remain unknown. As hypoxia inducible factor 1α (HIF-1α) is neuroprotective in stroke and mediates neuroinflammation, we tested the hypothesis that HIF-1α is a key factor of RIPC against stroke by mediating inflammation. Stroke was induced by transient middle cerebral artery occlusion in rats, and RIPC was conducted in both hind limbs. The HIF-1α mRNA was examined by quantitative reverse transcription polymerase chain reaction after RIPC. In addition, inflammatory cytokines in the peripheral blood and brain were measured using the AimPlex multiplex immunoassays. Data showed that RIPC reduced the infarct size, improved neurological functions, and increased HIF-1α mRNA levels, interleukin (IL)-4, and IL-10 protein levels in the peripheral blood. Intraperitoneal injection of the HIF activator, dimethyloxaloylglycine, reduced the infarct size and inhibited interferon-γ protein levels, while promoting IL-4 and IL-10 protein levels, while decreasing interferon-γ protein levels in both the peripheral blood and ischemic brain. In addition, injection of dimethyloxaloylglycine had a synergistic effect with ...Continue Reading

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Citations

Jan 1, 2019·Experimental Physiology·Justin D SprickCaroline A Rickards
Jan 14, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Felicity LillingstonRandall Waechter
Jan 28, 2020·International Journal of Molecular Medicine·Huizheng MaoMin Wang
May 8, 2018·International Journal of Molecular Sciences·Samuel J Geiseler, Cecilie Morland
Sep 12, 2018·Journal of Molecular Neuroscience : MN·Na LuHaixia Lu
Feb 6, 2020·Physical Therapy Research·Harutoshi Sakakima
Aug 9, 2020·International Journal of Molecular Sciences·Chiara Corrado, Simona Fontana
Feb 26, 2021·Basic Research in Cardiology·Lucie PearceDerek M Yellon
Jul 19, 2020·Journal of Neuroimmunology·Narayanappa AmrutaGregory Bix
Aug 8, 2021·International Journal of Molecular Sciences·Elena Rybnikova, Natalia Nalivaeva
Aug 12, 2021·General Thoracic and Cardiovascular Surgery·José García-de-la-AsunciónEva García-Del-Olmo
Aug 28, 2021·Frontiers in Cardiovascular Medicine·Emilio Y Lucero García RojasRichard A Bond

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Methods Mentioned

BETA
PCR

Software Mentioned

AimPlex
Prism
GraphPad
FCAP Array

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