Hypoxia-inducible factor-1α regulates epithelial-to-mesenchymal transition in paraquat-induced pulmonary fibrosis by activating lysyl oxidase

Experimental and Therapeutic Medicine
Jian LuRui-lan Wang

Abstract

Pulmonary fibrosis (PF) is one of the most prevalent causes of death following paraquat (PQ) poisoning. As demonstrated in previous studies by the present authors, epithelial-to-mesenchymal transition (EMT) is associated with PQ-induced PF. In addition, hypoxia-inducible factor-1α (HIF-1α) and lysyl oxidase (LOX) promote EMT following PQ poisoning. However, the association between HIF-1α- and LOX-mediated regulation of EMT remains unclear. The present study investigated the association between HIF-1α and LOX with regard to PQ-induced EMT. A549 and RLE-6TN cells were treated with PQ, and HIF-1α and LOX expression was silenced with short interfering RNAs. Changes in the expression of HIF-1α, LOX, β-catenin and EMT-related makers were detected using real-time quantitative polymerase chain reaction, immunofluorescence, and western blotting. HIF-1α and LOX were associated with PQ-induced EMT, and their expression levels were significantly increased (P<0.05). LOX expression was significantly decreased following PQ poisoning when HIF-1α expression was inhibited (P<0.05). However, the level of HIF-1α did not change significantly when LOX was silenced. The expression level of β-catenin and the degree of EMT were significantly decreased ...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 28, 2006·Nature·Janine T ErlerAmato J Giaccia
Dec 29, 2007·Critical Reviews in Toxicology·R J Dinis-OliveiraF Carvalho
Jun 3, 2009·The Journal of Clinical Investigation·Michael Zeisberg, Eric G Neilson
Oct 6, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Guofei ZhouJacob I Sznajder
May 18, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Begoña LópezJavier Díez
Aug 3, 2012·Current Opinion in Pulmonary Medicine·Hidenori Kage, Zea Borok
Dec 1, 2012·Cell Structure and Function·Takaaki M GotoHideyuki Saya
Dec 22, 2012·Nature Reviews. Cancer·Jamie N Anastas, Randall T Moon
Jan 25, 2013·Cancer Research·Thomas R CoxJanine T Erler
Sep 21, 2013·Experimental Biology and Medicine·Hui XieShan Gao
Jan 8, 2014·Drug and Chemical Toxicology·Lingjie XuZhong Wang
Feb 22, 2014·Nature Reviews. Molecular Cell Biology·Samy LamouilleRik Derynck
Oct 29, 2014·Journal of Molecular Cell Biology·Tao ChengGaoxiang Ge
Nov 20, 2014·Journal of Korean Medical Science·Hyo-wook GilSae-Yong Hong
Mar 19, 2015·International Journal of Cancer. Journal International Du Cancer·Kuppusamy Balamurugan
Nov 19, 2015·Acta Pharmaceutica Sinica. B·Georgina N Masoud, Wei Li
Jan 20, 2016·Journal of Cellular and Molecular Medicine·Yong ZhuRuilan Wang
Jul 2, 2016·Cell·M Angela NietoJean Paul Thiery
Jul 18, 2016·Cell and Tissue Research·Ian A Darby, Tim D Hewitson
Jul 28, 2016·Cell and Tissue Research·Rivka C StoneMarjana Tomic-Canic
Oct 16, 2016·Cancer Research·Caroline ReynaudPhilippe Clézardin
Nov 8, 2016·Scientific Reports·Bang-Li HuHai-Xing Jiang
Nov 10, 2016·PloS One·Ernesto Martinez-MartinezNatalia Lopez-Andres

❮ Previous
Next ❯

Citations

May 8, 2020·Oxidative Medicine and Cellular Longevity·Xiaoji CuiYing Liang
Jun 19, 2021·World Journal of Emergency Medicine·Jian-Hua YiYu-Wen Huang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
protein assay
transfection

Software Mentioned

SPSS
ImageJ

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.