Hypoxia-inducible factor-1alpha mediates oral squamous cell carcinoma invasion via upregulation of alpha5 integrin and fibronectin

Biochemical and Biophysical Research Communications
Mi Heon RyuHae Ryoun Park

Abstract

With progressive and rapid growth of malignant tumors, cancer cells in an ischemic condition are expected to develop an increased potential for local invasive growth. To address this hypothesis, we first examined the effect of hypoxia on the invasiveness of oral squamous cell carcinoma (OSCC) cells using the Matrigel invasion assay. We then investigated the effect of hypoxia on the protein and mRNA expression of alpha5 integrin and fibronectin, which are major factors involved in tumor cell invasion. We showed that (i) hypoxia increased the invasiveness of OSCC cells, (ii) alpha5 integrin and fibronectin protein and mRNA expression levels were increased in OSCC cells under hypoxic conditions, (iii) hypoxia stimulated autocrine secretion of fibronectin in OSCC cells, (iv) administration of siRNA(HIF-1alpha) caused a significant decrease in alpha5 integrin and fibronectin protein, confirming that HIF-1alpha plays a role in their induction, and (v) siRNA(HIF-1alpha) abrogated hypoxia-induced cell invasion. Collectively, these data suggest that hypoxia promotes OSCC cell invasion that is elicited by HIF-1alpha-dependent alpha5 integrin and fibronectin induction.

References

Mar 1, 1992·Clinical & Experimental Metastasis·K R GehlsenP Sriramarao
Sep 1, 1995·Cancer Metastasis Reviews·S K AkiyamaK M Yamada
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·P H MaxwellP J Ratcliffe
May 23, 2000·Critical Reviews in Biochemistry and Molecular Biology·G L Semenza
Apr 27, 2001·Seminars in Cancer Biology·A M Mercurio, I Rabinovitz
Apr 3, 2002·Trends in Molecular Medicine·Gregg L Semenza
Aug 30, 2003·Oncogene·Nicholas C DenkoAmato J Giaccia
Sep 18, 2003·Nature Reviews. Cancer·Gregg L Semenza
May 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tetsufumi KoikeReiji Kanangi

❮ Previous
Next ❯

Citations

Nov 13, 2012·Cancer Biotherapy & Radiopharmaceuticals·Ling WeiLi-Yan Lv
May 17, 2011·PloS One·Neil R HackettRonald G Crystal
Jun 6, 2015·Archives of Pathology & Laboratory Medicine·Lizandra JimenezJeffrey E Segall
Aug 8, 2014·BioMed Research International·Dongjun LuoJunhua Wu
Nov 3, 2012·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Ching-Yu YenHsueh-Wei Chang
Jul 3, 2015·Mediators of Inflammation·Azael Torales-CardeñaMario E Cancino-Díaz
Jul 5, 2012·European Journal of Pharmacology·Manoj NepalYunjo Soh
Oct 1, 2011·Cancer Letters·Mario Pérez-SayánsAbel García-García
Jan 21, 2017·International Journal of Molecular Sciences·Rehman Ata, Costin N Antonescu
Jan 10, 2012·Biotechnology Progress·Bhushan J ToleyNeil S Forbes
Dec 22, 2019·Cells·Tsung-Cheng LinHung-Chi Cheng
Mar 8, 2018·International Journal of Molecular Medicine·Yang DingJianmin Han
Oct 19, 2013·Oncology Letters·Karuza Maria Alves PereiraFabrício Bitu Sousa
Oct 4, 2019·Nigerian Journal of Surgery : Official Publication of the Nigerian Surgical Research Society·Abikshyeet PandaLipsa Bhuyan
Jan 1, 2021·International Journal of Molecular Sciences·Stephan Niland, Johannes A Eble
Jan 14, 2021·Journal of Cellular Biochemistry·Carina MagdalenoArchana Varadaraj
May 2, 2021·Scientific Reports·Shankargouda PatilAditi Chatterjee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.