Hypoxia-inducible factor-2α induces expression of type X collagen and matrix metalloproteinases 13 in osteoarthritic meniscal cells

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
Shinya IshizukaNaoki Ishiguro

Abstract

To evaluate whether Hypoxia-inducible factor-2α (HIF-2α) regulates expression of endochondral ossification-related molecules in human OA meniscus. Expressions of HIF-2α, type X collagen (COL10), matrix metalloproteinase (MMP)-13, and vascular endothelial growth factor (VEGF) in non-OA and OA menisci were analyzed by real-time RT-PCR and immunohistochemistry (IHC). Meniscal cells from OA patients were treated with interleukin-1β (IL-1β) and gene expression was analyzed. After knockdown of HIF-2α in OA meniscal cells, COL10 and MMP-13 expression were analyzed by RT-PCR, western blotting, immunofluorescence and ELISA. Histological analysis demonstrated weak staining of the superficial layer and large round cells in OA meniscus. RT-PCR analysis showed that HIF-2α, COL10, MMP-13, and VEGF mRNA expressions were higher in OA than non-OA meniscal cells. IHC showed a coordinated staining pattern of HIF-2α, COL10, and MMP-13 in OA meniscus. IL-1β treatment increased HIF-2α, COL10, and MMP-13 expressions in OA meniscal cells, and knockdown of HIF-2α suppressed IL-1β-mediated increase in COL10 and MMP-13 expression. These results suggested that HIF-2α may cause meniscal matrix degradation by transactivation of MMP-13. HIF-2α may be a thera...Continue Reading

References

Nov 1, 1989·The Journal of Cell Biology·A R Poole, I Pidoux
Nov 1, 1989·The Journal of Cell Biology·P BrucknerM A Spycher
Jan 1, 1983·The Journal of Bone and Joint Surgery. British Volume·N R FahmyJ Noble
Mar 1, 1982·The American Journal of Sports Medicine·S P Arnoczky, R F Warren
Jan 19, 1996·The Journal of Biological Chemistry·V KnäuperG Murphy
May 20, 1998·Arthritis and Rheumatism·J L HuebnerV B Kraus
Sep 18, 1999·Osteoarthritis and Cartilage·G BluteauF Mallein-Gerin
Aug 30, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·B CatersonC B Little
Sep 12, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·M P Hellio Le GraverandD A Hart
Aug 18, 2001·Arthritis and Rheumatism·M P Hellio Le GraverandJ B Rattner
Sep 27, 2005·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Christopher B ForsythRichard F Loeser
Mar 4, 2006·Journal of Biomedical Materials Research. Part a·Sun-Woong KangByung-Soo Kim
Jan 9, 2007·Osteoarthritis and Cartilage·A K LangeB Vanwanseele
Jan 25, 2007·The American Journal of Sports Medicine·Ozgur Ahmet AtayDarren L Johnson
Feb 9, 2008·Cell Death and Differentiation·S A Patel, M C Simon
Feb 28, 2009·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Anestis PapadopoulosGeorge A Kapetanos
Oct 30, 2009·Connective Tissue Research·Zhenyu TangK-L Paul Sung
Nov 19, 2009·Arthritis Research & Therapy·Christopher G WilsonMarc E Levenston
Apr 1, 2010·Arthritis Research & Therapy·Yubo SunHelen E Gruber
Feb 18, 2011·Osteoarthritis and Cartilage·W B van den Berg
May 17, 2011·Molecular Cancer Research : MCR·Qun LinZhong Yun
Aug 16, 2011·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Hideki HiraiwaNaoki Ishiguro
May 20, 2014·Arthritis & Rheumatology·Michael D RushtonJohn Loughlin

❮ Previous
Next ❯

Citations

Aug 23, 2016·Oxidative Medicine and Cellular Longevity·Xinfeng ChenYi Zhang
Mar 9, 2020·Journal of Bone and Mineral Metabolism·Lian WangTao Li
Jul 19, 2018·Rheumatology International·Elena V Tchetina, Galina A Markova
Aug 30, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yan ZhouShiqing Liu
Oct 6, 2020·JACC. Basic to Translational Science·Milton Packer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Related Papers

Biochemical and Biophysical Research Communications
Motoshige NakashimaNaoki Ishiguro
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Wei JiangChao Zeng
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Takehiko MatsushitaRyosuke Kuroda
© 2022 Meta ULC. All rights reserved