Hypoxia inhibits human bladder smooth muscle cell proliferation: a potential mechanism of bladder dysfunction

Neurourology and Urodynamics
D J GalvinJ M Fitzpatrick

Abstract

Recent animal studies have suggested that bladder outflow obstruction causes bladder wall hypoxia during both the filling and the voiding phases of the micturition cycle. We have previously demonstrated that mechanical deformation of human detrusor leads to smooth muscle (SM) cell hypertrophy and hyperplasia, which may then contribute to hypoxia in the dysfunctional bladder. We hypothesise that the detrusor's response to a hypoxic environment contributes to bladder dysfunction. The aim of this study was to evaluate the effect of hypoxia on detrusor cell survival and growth. Normal human detrusor muscle was obtained at radical cystectomy and primary cultures were established. Cells were then cultured in the presence of 1% oxygen in a hypoxic chamber for different times. Apoptosis was assessed by propidium iodide DNA staining and flow cytometry. Proliferation was assessed by radiolabelled thymidine incorporation. Cell supernatants were retained for growth factor estimation by enzyme linked immuno-sorbent assay (ELISA), and total cell and nuclear extracts were isolated for Western blotting. SM cells responded to the presence of hypoxia through significant upregulation of survival factors hypoxia inducible factor (HIF 1alpha) and v...Continue Reading

References

Feb 1, 1975·British Journal of Urology·M Dunn
Oct 1, 1990·Cell and Tissue Research·G Gabella, B Uvelius
Apr 26, 2000·American Journal of Physiology. Endocrinology and Metabolism·Z LinF C Brosius
Aug 12, 2000·Molecular and Cellular Biochemistry·P ChichesterR M Levin
Oct 6, 2000·The Journal of Clinical Investigation·G L Semenza
Dec 23, 2000·The Journal of Urology·R N CoffeyJ M Fitzpatrick
Feb 15, 2001·The Journal of Urology·A SchröderR M Levin
Feb 8, 2002·The Journal of Urology·Mohamed A GhafarRalph Buttyan
Feb 28, 2002·Biochemical and Biophysical Research Communications·Louis X LiuCanwen Jiang
Jul 16, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Mohamed A GhafarRobert M Levin
Oct 22, 2002·American Journal of Physiology. Renal Physiology·David J GalvinJohn M Fitzpatrick

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Citations

Aug 25, 2007·International Journal of Impotence Research·A F BradingH Hashitani
Mar 11, 2008·Investigative Ophthalmology & Visual Science·Ying-Bo Shui, David C Beebe
Aug 19, 2009·The Journal of Sexual Medicine·Raanan TalJohn P Mulhall
Jul 17, 2009·Differentiation; Research in Biological Diversity·Unai SilvánJuan Aréchaga
Jan 4, 2017·Therapeutic Advances in Urology·Karl-Erik AnderssonAxel Forman
Oct 14, 2017·Annals of Clinical Biochemistry·John W Honour
Apr 4, 2008·BJU International·George KoritsiadisConstantinos Constantinides
Feb 27, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Weilin ZhouJ Usha Raj
Jul 7, 2019·Neurourology and Urodynamics·Ruud BoschEskinder Solomon
Jul 1, 2016·American Journal of Physiology. Renal Physiology·Vittore VerrattiAnna Maria Aloisi
Nov 7, 2012·International Journal of Urology : Official Journal of the Japanese Urological Association·Yun Seob SongSeung U Kim
Dec 4, 2020·International Neurourology Journal·Su Jin Kim

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