Hypoxia modulates cell migration and proliferation in placenta-derived mesenchymal stem cells

The Journal of Thoracic and Cardiovascular Surgery
Li LiRenzo Cecere

Abstract

For more than a decade, stem cells isolated from different tissues have been evaluated in cell therapy. Among them, the human bone marrow-derived mesenchymal stem cells (hBM-MSCs) were investigated extensively in the treatment of myocardial infarction. Recently, the human placenta-derived mesenchymal stem cells (hPD-MSCs), which are readily available from a biological waste, appear to be a viable alternative to hBM-MSCs. C-X-C chemokine receptor type 4 (CXCR4) gene expression and localization were detected and validated in hPD-MSCs and hBM-MSCs via polymerase chain reaction and immunofluorescence. Subsequently, cell culture conditions for CXCR4 expression were optimized in stromal-derived factor-1 alpha (SDF1-α), glucose, and cobalt chloride (CoCl2) by the use of cell viability, proliferation, and migration assays. To elucidate the cell signaling pathway, protein expression of CXCR4, hypoxia-inducible factor-1α, interleukin-6, Akt, and extracellular signal-regulated kinase were analyzed by Western blot. CXCR4-positive cells were sorted and analyzed by florescence-activated cell sorting. CXCR4 was expressed on both hPD-MSCs and hBM-MSCs at the basal level. HPD-MSCs were shown to have a greater sensitivity to SDF-1α-dependent cel...Continue Reading

Citations

Feb 1, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dun Xian TanRussel J Reiter
Aug 18, 2018·Journal of Cellular and Molecular Medicine·Gang LiGui-Rong Li
Aug 9, 2019·Cell Biology International·Najva AlijaniMehdi Kadivar
Mar 15, 2018·Stem Cells International·Heiko LemckeRobert David
Aug 28, 2020·Frontiers in Immunology·Siyu LiuShu Guo
Nov 3, 2021·Journal of Tissue Engineering and Regenerative Medicine·Kashif KhanRenzo Cecere

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