Hypoxia modulates the development of a corneal stromal matrix model

Experimental Eye Research
Albert LeeVickery Trinkaus-Randall

Abstract

Deposition of matrix proteins during development and repair is critical to the transparency of the cornea. While many cells respond to a hypoxic state that can occur in a tumor, the cornea is exposed to hypoxia during development prior to eyelid opening and during the diurnal sleep cycle where oxygen levels can drop from 21% to 8%. In this study, we used 2 three-dimensional (3-D) models to examine how stromal cells respond to periods of acute hypoxic states. The first model, a stromal construct model, is a 3-D stroma-like construct that consists of human corneal fibroblasts (HCFs) stimulated by a stable form of ascorbate for 1, 2, and 4 weeks to self-assemble their own extracellular matrix. The second model, a corneal organ culture model, is a corneal wound-healing model, which consists of wounded adult rat corneas that were removed and placed in culture to heal. Both models were exposed to either normoxic or hypoxic conditions for varying time periods, and the expression and/or localization of matrix proteins was assessed. No significant changes were detected in Type V collagen, which is associated with Type I collagen fibrils; however, significant changes were detected in the expression of both the small leucine-rich repeatin...Continue Reading

Citations

Mar 13, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Obianamma E OnochieVickery Trinkaus-Randall
Feb 13, 2020·Bioengineering·Peiluo XuVickery Trinkaus-Randall
Sep 29, 2020·Current Protocols in Cell Biology·Tina B McKayJoseph B Ciolino
Jul 25, 2020·Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)·Alejandra ConsejoStephen J Vincent
Jul 4, 2020·Experimental Eye Research·Tina B McKayDimitrios Karamichos
Mar 31, 2021·Npj Regenerative Medicine·Andrea De PieriDimitrios I Zeugolis

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