Hypoxia precondition promotes adipose-derived mesenchymal stem cells based repair of diabetic erectile dysfunction via augmenting angiogenesis and neuroprotection

PloS One
XiYou WangMingHui Yang

Abstract

The aim of the present study was to examine whether hypoxia preconditioning could improve therapeutic effects of adipose derived mesenchymal stem cells (AMSCs) for diabetes induced erectile dysfunction (DED). AMSCs were pretreated with normoxia (20% O2, N-AMSCs) or sub-lethal hypoxia (1% O2, H-AMSCs). The hypoxia exposure up-regulated the expression of several angiogenesis and neuroprotection related cytokines in AMSCs, including vascular endothelial growth factor (VEGF) and its receptor FIK-1, angiotensin (Ang-1), basic fibroblast growth factor (bFGF), brain-derived neurotrophic factor (BDNF), glial cell-derived neurotrophic factor (GDNF), stromal derived factor-1 (SDF-1) and its CXC chemokine receptor 4 (CXCR4). DED rats were induced via intraperitoneal injection of streptozotocin (60 mg/kg) and were randomly divided into three groups-Saline group: intracavernous injection with phosphate buffer saline; N-AMSCs group: N-AMSCs injection; H-AMSCs group: H-AMSCs injection. Ten rats without any treatment were used as normal control. Four weeks after injection, the mean arterial pressure (MAP) and intracavernosal pressure (ICP) were measured. The contents of endothelial, smooth muscle, dorsal nerve in cavernoursal tissue were asses...Continue Reading

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Citations

Jan 14, 2016·Current Urology Reports·Ahmet GokceWayne J Hellstrom
Mar 1, 2016·Kidney International·LaTonya J HicksonLilach O Lerman
Jan 14, 2016·Current Opinion in Urology·M Ayodhia SoebadiMaarten Albersen
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Aug 24, 2017·Journal of Cellular and Molecular Medicine·Stephanie Merfeld-ClaussDmitry O Traktuev
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Sep 24, 2020·Journal of Materials Science. Materials in Medicine·Qiong LiGuodong Wang
Feb 13, 2019·Tissue Engineering. Part C, Methods·Amita AjitLissy K Krishnan
Apr 19, 2017·International Urology and Nephrology·Quan-Liang HouWei-Dong Zhang
Feb 3, 2018·Journal of Cellular and Molecular Medicine·Chenxia Hu, Lanjuan Li
Nov 20, 2020·Scientific Reports·Joanna BukowskaBarbara Gawrońska-Kozak
May 18, 2021·Frontiers in Cell and Developmental Biology·Szu-Hsien WuJung-Pan Wang
Nov 10, 2018·ACS Chemical Neuroscience·Feng ZhangWeidong Le

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Methods Mentioned

BETA
fluorescence-activated cell sorting
PCR
electrophoresis
Protein Assay
flow cytometry
flowcytometry

Software Mentioned

ImagePro

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