Hypoxia promotes bone marrow-derived mesenchymal stem cell proliferation through apelin/APJ/autophagy pathway

Acta Biochimica Et Biophysica Sinica
Lanfang LiZhisheng Jiang

Abstract

Bone marrow-derived mesenchymal stem cells (BMSCs) are a population of multipotent progenitors that have the capacity of proliferation and differentiation into mesenchymal lineage cells. The regulatory peptide apelin is the endogenous ligand for the G protein-coupled receptor APJ. Apelin, which can enhance BMSC proliferation, has mitogenic effects on a wide variety of cell types. We hypothesized that the increased apelin/APJ might be involved in the occurrence and development of hypoxia-induced BMSC proliferation. BMSCs from the bone marrow of 8- to 10-week-old C57BL/6J mice were cultured under either normoxia (21% oxygen) or hypoxia (1% oxygen) condition. Cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and 5-bromo-2'-deoxyuridine assay. Expressions of hypoxia-inducible factor (HIF)-1α, apelin, APJ, Beclin-1, and LC3II/LC3I were detected by western blot analysis. Results suggested that hypoxia enhanced the proliferation of BMSC in a time-dependent manner. The expressions of HIF-1α, apelin, APJ, Beclin-1, and LC3II/LC3I were increased in BMSCs induced by hypoxia. Small interfering RNA (siRNA)-HIF-1α that inhibited the hypoxia-induced expressions of apelin, APJ, Beclin-1, an...Continue Reading

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Citations

Oct 6, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Lu HeLanfang Li
Feb 27, 2016·Clinical and Experimental Pharmacology & Physiology·Deguan LvLinxi Chen
Apr 17, 2016·Acta Biochimica Et Biophysica Sinica·Lanfang LiZhisheng Jiang
Jun 6, 2019·Cell Biology International·Shahnaz EsmaeiliEnsieh Nasli-Esfahani
Nov 5, 2019·The Journal of Physiological Sciences : JPS·Ramada R KhasawnehHadeel Abu-El-Rub
Jun 8, 2018·Frontiers in Physiology·Marta B WysockaDorota Nowak
May 23, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jelena JakovljevicVladislav Volarevic
Jun 15, 2020·Ageing Research Reviews·Xu LiLing Qin
Feb 23, 2020·Advances in Medical Sciences·Javad MasoumiNajmeh Jamali
Mar 7, 2021·International Journal of Molecular Sciences·Silvia PeregoGiovanni Lombardi

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