Abstract
Bone marrow-derived mesenchymal stem cells (BMSCs) are a population of multipotent progenitors that have the capacity of proliferation and differentiation into mesenchymal lineage cells. The regulatory peptide apelin is the endogenous ligand for the G protein-coupled receptor APJ. Apelin, which can enhance BMSC proliferation, has mitogenic effects on a wide variety of cell types. We hypothesized that the increased apelin/APJ might be involved in the occurrence and development of hypoxia-induced BMSC proliferation. BMSCs from the bone marrow of 8- to 10-week-old C57BL/6J mice were cultured under either normoxia (21% oxygen) or hypoxia (1% oxygen) condition. Cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and 5-bromo-2'-deoxyuridine assay. Expressions of hypoxia-inducible factor (HIF)-1α, apelin, APJ, Beclin-1, and LC3II/LC3I were detected by western blot analysis. Results suggested that hypoxia enhanced the proliferation of BMSC in a time-dependent manner. The expressions of HIF-1α, apelin, APJ, Beclin-1, and LC3II/LC3I were increased in BMSCs induced by hypoxia. Small interfering RNA (siRNA)-HIF-1α that inhibited the hypoxia-induced expressions of apelin, APJ, Beclin-1, an...Continue Reading
References
Oct 30, 1998·Biochemical and Biophysical Research Communications·K TatemotoM Fujino
Jan 10, 2002·Biochemical and Biophysical Research Communications·Bernard MasriYves Audigier
Dec 13, 2003·Endocrinology·Guiyun WangGeorge H Greeley
Oct 11, 2007·Aging Cell·Christine FehrerGünter Lepperdinger
Mar 28, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Song HanGeorge H Greeley
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Feng LiLinxi Chen
Jul 12, 2008·Circulation Research·Mélanie EyriesFlorent Soubrier
Sep 22, 2009·American Journal of Physiology. Heart and Circulatory Physiology·David N CharoThomas Quertermous
Mar 31, 2011·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·K GeigerH Drexel
Apr 5, 2011·Critical Care : the Official Journal of the Critical Care Forum·Samuel N HeymanChristian Rosenberger
Jul 4, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Lanfang LiJian-Xiong Chen
Apr 17, 2013·Acta Biochimica Et Biophysica Sinica·Deguan LvLinxi Chen
Aug 9, 2013·Acta Biochimica Et Biophysica Sinica·Lifang LiJian Li
Sep 17, 2013·PloS One·Lanfang LiJian-Xiong Chen
Nov 14, 2013·Autophagy·Xiao-Wen LiuBo Yang
Jan 1, 2014·Acta Biochimica Et Biophysica Sinica·Li YangLinxi Chen
Jun 27, 2014·Acta Biochimica Et Biophysica Sinica·Feng XieLinxi Chen
Citations
Feb 20, 2016·Life Sciences·Lili DuJing Xu
Oct 6, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Lu HeLanfang Li
Jan 16, 2016·Molecular and Cellular Endocrinology·Pieter-Jan StiersGeert Carmeliet
Feb 27, 2016·Clinical and Experimental Pharmacology & Physiology·Deguan LvLinxi Chen
Apr 17, 2016·Acta Biochimica Et Biophysica Sinica·Lanfang LiZhisheng Jiang
Feb 24, 2016·Scientific Reports·Dong LiangFeng Cao
Feb 24, 2018·Stem Cell Research & Therapy·Jianming GuoYongquan Gu
May 19, 2019·Cells·Carl Randall HarrellVladislav Volarevic
Jun 6, 2019·Cell Biology International·Shahnaz EsmaeiliEnsieh Nasli-Esfahani
Nov 16, 2019·Cellular Reprogramming·Xiaoqin WangXiaojiao Huang
Oct 1, 2017·Stem Cell Research & Therapy·Jingying HouTong Wang
Apr 19, 2019·Stem Cell Research & Therapy·Chenxia HuLanjuan Li
Oct 17, 2015·The Journal of Biological Chemistry·Hong-Zheng MengMao-Wei Yang
Nov 5, 2019·The Journal of Physiological Sciences : JPS·Ramada R KhasawnehHadeel Abu-El-Rub
May 17, 2018·Stem Cells International·Xihang ChenFeng Lu
Jun 8, 2018·Frontiers in Physiology·Marta B WysockaDorota Nowak
Nov 7, 2019·Stem Cells International·Zsolt Fábián
May 23, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jelena JakovljevicVladislav Volarevic
Jun 15, 2020·Ageing Research Reviews·Xu LiLing Qin
Jan 31, 2020·Cytokine·Chiman MohammadiRezvan Najafi
Feb 23, 2020·Advances in Medical Sciences·Javad MasoumiNajmeh Jamali
Mar 7, 2021·International Journal of Molecular Sciences·Silvia PeregoGiovanni Lombardi
Mar 20, 2021·Frontiers in Cell and Developmental Biology·Hao ZhangYuelin Zhang
Jun 4, 2021·Scientific Reports·Xiaobo Guo, Zhenyuan Wu