Abstract
Adipose-derived stem cell (ADSC) is an alternative and less invasive source of mesenchymal stem cells which can be used to develop biological treatment strategies for tissue regeneration, and their therapeutic applications hinge on an understanding of their physiological characteristics. N6-Methyladenosine (m6A) is the most common chemical modification of mRNAs and has recently been revealed to play important roles in cell lineage differentiation and development. However, the role of m6A modification in the vascular smooth muscle cell (VSMC) differentiation of ADSCs remains unclear. Herein, we investigated the expression of N6-adenosine methyltransferases (Mettl3) and demethylases (Fto and Alkbh5) and found that Mettl3 was upregulated in ADSCs undergoing vascular smooth muscle differentiation induction. Moreover, silence of Mettle3 reduced the expression level of VSMC-specific markers, including α-SMA, SM22α, calponin, and SM-MHC. Meanwhile, Mettl3 knockdown also decreased the expression of paracrine factors, including VEGF, HGF, TGF-β, GM-CSF, bFGF, and SDF-1. In addition, our results suggested that hypoxia stress promotes the ADSC differentiate into VMSCs and regulates the secretion of VEGF, HGF, TGF-β, GM-CSF, bFGF, and SDF-...Continue Reading
References
Aug 4, 2001·Circulation Research·K HayashiK Sobue
Nov 29, 2005·Cellular Signalling·Sheng-Chieh LienJeng-Jiann Chiu
Oct 20, 2007·The Journal of Biological Chemistry·Giorgio LagnaAkiko Hata
Nov 28, 2009·Current Stem Cell Research & Therapy·Hiroshi Mizuno
May 23, 2012·Cell·Kate D MeyerSamie R Jaffrey
Dec 12, 2012·Trends in Genetics : TIG·Guifang JiaChuan He
Mar 5, 2013·Cellular and Molecular Life Sciences : CMLS·Fábio G TeixeiraAntónio J Salgado
Jan 11, 2014·Cell Research·Xiao-Li PingYun-Gui Yang
May 7, 2014·Cytotechnology·Caterina FotiaDonatella Granchi
Sep 23, 2014·Nature Chemical Biology·Chao XuJinrong Min
Dec 3, 2014·Cell Stem Cell·Pedro J BatistaHoward Y Chang
Jan 9, 2015·Science·Shay GeulaJacob H Hanna
Jun 6, 2015·Cell·Xiao WangChuan He
Jun 30, 2015·Nature Methods·Bastian LinderSamie R Jaffrey
Jul 15, 2015·Genes & Development·Yanan YueChuan He
Aug 8, 2015·Journal of Tissue Engineering and Regenerative Medicine·Xiaowei WuRan Xiao
Nov 13, 2015·Stem Cell Research & Therapy·Natalia KalininaVadim Govorun
Feb 18, 2016·Stem Cells International·Kevin DzoboM Iqbal Parker
Feb 21, 2016·Nucleic Acids Research·Yuan ZhouQinghua Cui
Mar 24, 2016·Proceedings of the National Academy of Sciences of the United States of America·Chuanzhao ZhangGregg L Semenza
Jun 15, 2016·Stem Cell Research & Therapy·Wojciech WystrychowskiRamin E Beygui
Jul 5, 2016·Molecular Cell·Ping WangYunsun Nam
Aug 17, 2016·International Journal of Molecular Sciences·Kevin DzoboM Iqbal Parker
Aug 26, 2016·Nature Communications·Hao DuLigang Wu
Aug 27, 2016·Stem Cell Research & Therapy·Everardus J van ZoelenSebastian P van der Woning
Sep 30, 2016·Cell Proliferation·Yang YuFa-Ming Chen
Jan 13, 2017·Genome Biology·Thomas AmortAlexandra Lusser
Jan 24, 2017·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Egon BurianSven Otto
Feb 7, 2017·Connective Tissue Research·Arina T BuizerRoel Kuijer
Jun 24, 2017·Genes & Development·Shengdong KeRobert B Darnell
Aug 16, 2017·Cell Research·Kai XuWei Li
Sep 20, 2017·Proceedings of the National Academy of Sciences of the United States of America·Mireya Martínez-PérezVicente Pallás
Oct 7, 2017·ELife·Ian A RoundtreeChuan He
Dec 11, 2017·Biochemistry. Biokhimii︠a︡·Yongwei LiJitao Wu
Jan 18, 2018·Nature Neuroscience·Yang WangJing Crystal Zhao
Feb 15, 2018·Die Pharmazie·Yongwei LiJitao Wu
Jun 8, 2018·PLoS Biology·Chen-Xin WangQi Zhou
Jul 27, 2018·Neuron·Mareen EngelAlon Chen
Jul 31, 2018·Neuron·Jessica L KorandaXiaoxi Zhuang
Jan 1, 2017·Molecular & Cellular Biomechanics : MCB·Ruikai Chen, Delphine Dean
Jan 31, 2019·International Journal of Molecular Sciences·Cheng TianQiong Xu
May 8, 2019·Nature Cell Biology·Heather LeeLei Ding
Aug 11, 2019·Journal of Translational Medicine·Rida Al-RifaiClaire Tournois
Citations
Sep 23, 2020·Nutrition and Cancer·Chen WangQihong Zhao
Mar 7, 2021·International Journal of Molecular Sciences·Emma WilkinsonYu-Ying He
Mar 21, 2021·Life Sciences·Yuhan QinGaoliang Yan
May 25, 2021·Frontiers in Cardiovascular Medicine·Dandan SongJunnan Wang
Jul 6, 2021·Oxidative Medicine and Cellular Longevity·Jun ChenDing-Sheng Jiang
Jul 1, 2021·Tissue Engineering. Part B, Reviews·Xun XuWeidong Tian
Aug 10, 2021·Frontiers in Cell and Developmental Biology·Wenhao JuJianxin Wu
Aug 13, 2021·Journal of Cellular and Molecular Medicine·Fei YeZhihui Zhang
Oct 9, 2021·Scientific Reports·Bartosz SikoraJoanna Gola
Oct 19, 2021·Frontiers in Cell and Developmental Biology·Shu-Juan XieZhen-Dong Xiao
Nov 2, 2021·Frontiers in Cell and Developmental Biology·Shu-Juan XieZhen-Dong Xiao