Hypoxia Promotes Vascular Smooth Muscle Cell (VSMC) Differentiation of Adipose-Derived Stem Cell (ADSC) by Regulating Mettl3 and Paracrine Factors

Stem Cells International
Jiaying LinYanping Kuang

Abstract

Adipose-derived stem cell (ADSC) is an alternative and less invasive source of mesenchymal stem cells which can be used to develop biological treatment strategies for tissue regeneration, and their therapeutic applications hinge on an understanding of their physiological characteristics. N6-Methyladenosine (m6A) is the most common chemical modification of mRNAs and has recently been revealed to play important roles in cell lineage differentiation and development. However, the role of m6A modification in the vascular smooth muscle cell (VSMC) differentiation of ADSCs remains unclear. Herein, we investigated the expression of N6-adenosine methyltransferases (Mettl3) and demethylases (Fto and Alkbh5) and found that Mettl3 was upregulated in ADSCs undergoing vascular smooth muscle differentiation induction. Moreover, silence of Mettle3 reduced the expression level of VSMC-specific markers, including α-SMA, SM22α, calponin, and SM-MHC. Meanwhile, Mettl3 knockdown also decreased the expression of paracrine factors, including VEGF, HGF, TGF-β, GM-CSF, bFGF, and SDF-1. In addition, our results suggested that hypoxia stress promotes the ADSC differentiate into VMSCs and regulates the secretion of VEGF, HGF, TGF-β, GM-CSF, bFGF, and SDF-...Continue Reading

References

Nov 28, 2009·Current Stem Cell Research & Therapy·Hiroshi Mizuno
Dec 12, 2012·Trends in Genetics : TIG·Guifang JiaChuan He
Mar 5, 2013·Cellular and Molecular Life Sciences : CMLS·Fábio G TeixeiraAntónio J Salgado
Sep 23, 2014·Nature Chemical Biology·Chao XuJinrong Min
Dec 3, 2014·Cell Stem Cell·Pedro J BatistaHoward Y Chang
Jun 30, 2015·Nature Methods·Bastian LinderSamie R Jaffrey
Nov 13, 2015·Stem Cell Research & Therapy·Natalia KalininaVadim Govorun
Mar 24, 2016·Proceedings of the National Academy of Sciences of the United States of America·Chuanzhao ZhangGregg L Semenza
Jun 15, 2016·Stem Cell Research & Therapy·Wojciech WystrychowskiRamin E Beygui
Jan 24, 2017·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Egon BurianSven Otto
Sep 20, 2017·Proceedings of the National Academy of Sciences of the United States of America·Mireya Martínez-PérezVicente Pallás
Jul 27, 2018·Neuron·Mareen EngelAlon Chen
Jan 1, 2017·Molecular & Cellular Biomechanics : MCB·Ruikai Chen, Delphine Dean
Jan 31, 2019·International Journal of Molecular Sciences·Cheng TianQiong Xu

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Citations

Mar 7, 2021·International Journal of Molecular Sciences·Emma WilkinsonYu-Ying He
May 25, 2021·Frontiers in Cardiovascular Medicine·Dandan SongJunnan Wang
Jul 6, 2021·Oxidative Medicine and Cellular Longevity·Jun ChenDing-Sheng Jiang
Jul 1, 2021·Tissue Engineering. Part B, Reviews·Xun XuWeidong Tian
Oct 9, 2021·Scientific Reports·Bartosz SikoraJoanna Gola
Nov 2, 2021·Frontiers in Cell and Developmental Biology·Shu-Juan XieZhen-Dong Xiao

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Methods Mentioned

BETA
flow cytometry
transfections
transfection
PCR
enzyme-linked immunosorbent assay

Software Mentioned

Stata SE15

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