PMID: 9551744Apr 29, 1998Paper

Hypoxia reduces endothelin production by rat alveolar type II cells in primary culture

The European Respiratory Journal
B CrestaniM Aubier

Abstract

The purpose of the study was to describe endothelin (ET) production and to characterize the effect of hypoxia on preproendothelin-1 (preproET-1) messenger ribonucleic acid (mRNA) expression and ET secretion by rat type II pneumocytes in vitro. Rat type II pneumocytes were incubated in a sealed chamber containing a normoxic (21% O2) or hypoxic (1% O2) atmosphere for increasing durations. Immunoreactive ET (irET) was measured in cell supernatants using a radioimmunoassay. Rat preproET-1 mRNA was detected by Northern blot. Rat type II pneumocytes expressed preproET-1 mRNA, contained irET and secreted irET in a time-dependent manner. ET secretion was dependent on de novo ribonucleic acid (RNA) and protein synthesis. Hypoxia decreased irET secretion by 27% and reduced the steady-state level of preproET-1 mRNA by 60% whereas intracellular irET concentration was unchanged. Inhibition was partially reversible with the return to a normoxic atmosphere. Inhibition of nitric oxide synthesis did not prevent the inhibitory effect of hypoxia. In conclusion, rat type II pneumocytes in primary culture secreted immunoreactive endothelin and expressed preproendothelin-1 messenger ribonucleic acid. Hypoxia reversibly reduced endothelin-1 productio...Continue Reading

Citations

Oct 18, 2007·American Journal of Respiratory Cell and Molecular Biology·Bruno Crestani
May 8, 2010·American Journal of Respiratory Cell and Molecular Biology·Carmen FonsecaElisabetta A Renzoni
Mar 25, 2000·The Journal of Thoracic and Cardiovascular Surgery·T L WeigelC Odoux
Jun 1, 2008·Expert Review of Respiratory Medicine·Jeffrey J Swigris, Kevin K Brown
Sep 19, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L Guembe, A C Villaro
Jul 10, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jose F Perez-Zoghbi, Michael J Sanderson

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