Hypoxia regulates vascular endothelial growth factor and soluble fms-like tyrosine kinase-1 secretion by human oviductal epithelial cells and stromal fibroblasts

Fertility and Sterility
Hiroko ItohHisashi Narahara

Abstract

To evaluate the effect of hypoxia on the production of vascular endothelial growth factor (VEGF) and soluble fms-like tyrosine kinase-1 (sFlt-1) in the human fallopian tube. The secretion of VEGF and sFlt-1 by cultured oviductal epithelial cells (OECs) and oviductal stromal fibroblasts (OSFs) in response to hypoxia was investigated. Research laboratory at a medical school. Normal oviducts obtained from seven premenopausal patients were used. Oviductal epithelial cells and OSFs were incubated under normoxic (20% O2) or hypoxic (2% O2) conditions. The concentrations of VEGF and sFlt-1 in the culture media of OECs and OSFs were measured by enzyme-linked immunosorbent assays. The secretion of both VEGF and sFlt-1 was detected in cultured OECs and OSFs and was found to have been stimulated under hypoxic conditions in these cells. The present findings suggest that hypoxia in the local environment may stimulate oviductal vascular permeability by inducing the production of VEGF by oviductal cells. Simultaneous up-regulation of sFlt-1 secretion by these cells under hypoxic conditions may prevent excessive up-regulation of vascular permeability. The modulation of the bias of VEGF and sFlt-1 in the fallopian tubes may contribute to the no...Continue Reading

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Citations

Oct 22, 2013·The Journal of Thoracic and Cardiovascular Surgery·Kurt R SchumacherJohn R Charpie
Feb 9, 2007·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Hiroko ItohHisashi Narahara
Apr 25, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ville WarisYrjö T Konttinen
Oct 31, 2006·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Kaei NasuHisashi Narahara
Sep 6, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Sergio GhersevichCarlos Zumoffen
Aug 28, 2021·Biomedicines·Veronika AleksandrovychKrzysztof Gil

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