Hypoxia-reoxygenation is as damaging as ischemia-reperfusion in the rat liver

Critical Care Medicine
S TanD A Parks

Abstract

We hypothesized that the extent of injury and release of xanthine oxidase, an oxidant generator, into the circulation would be less in normal-flow hypoxia-reoxygenation than in equal duration no-flow ischemia-reperfusion. Randomized study. University-based animal research facility. Male Sprague-Dawley rats. The livers were isolated, perfused, and then randomly subjected to 2 hrs of hypoxia (normal flow, low oxygen) or ischemia (no flow, no oxygen), and 2 hrs of reperfusion. Hepatocytes were also isolated, and were subjected to either: a) hypoxia (0, 2, 4, and 6 hrs); or b) hypoxia (2 and 4 hrs) with reoxygenation (2 hrs). The extent of liver injury (as assessed by release of hepatocellular enzymes) and the release of xanthine oxidase were measured from isolated-perfused rat livers and cultured hepatocytes. The pattern of release of xanthine oxidase in isolated-perfused liver effluent was different in hypoxia-reoxygenation compared with ischemia-reperfusion. During hypoxia, xanthine oxidase gradually increased in the effluent; then, the xanthine oxidase decreased to low concentrations during reoxygenation. After ischemia, there was a sharp spike in xanthine oxidase at 1 min of reperfusion, with a rapid decrease to low concentrat...Continue Reading

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Citations

Jul 11, 1998·Critical Care Medicine·P S Barie
Dec 24, 2005·Critical Care Medicine·Julien PottecherJacques Duranteau
Sep 22, 2012·Gynécologie, obstétrique & fertilité·G KayemB Haddad
Jan 1, 2008·The Journal of Surgical Research·Thomas A BroughanT Kent Teague
Jul 24, 2002·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·K AtilaI Astarcioglu
Jun 3, 2014·Biochimica Et Biophysica Acta·Maria Giulia BattelliLetizia Polito
Jun 19, 2002·Journal of Applied Physiology·Bob H M HeijnenThomas M Van Gulik

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