Hypoxia, RONS and energy metabolism in articular cartilage.

Osteoarthritis and Cartilage
B FermorB O Diekman

Abstract

Increased pro-inflammatory cytokines and reactive oxygen and nitrogen species (RONS) occur in osteoarthritis (OA). Oxygen tension can alter the levels of RONS induced by interleukin-1 (IL-1). RONS such as nitric oxide (NO) can alter energy metabolism. The aim of this study was to determine if oxygen tension alters energy metabolism, in articular cartilage, in response to IL-1 or NO and to determine if cell death occurred. Porcine articular chondrocytes were incubated with IL-1 or the NO donor NOC-18 for 48 h in either 1, 5 or 20% O(2). Adenosine triphosphate (ATP) levels were measured and immunoblots for adenosine monophosphate-activated protein kinase (AMPK) were done. Protein translation was measured by S6 activation. Senescence and autophagy were determined by increased caveolin or conversion of LC3-I to LC3-II respectively. One percent O(2) significantly reduced ATP levels compared with 20% O(2). Five percent O(2) significantly increased ATP levels compared with 20% O(2). One percent O(2) significantly increased phospho-AMPK (pAMPK) protein expression compared with 5 or 20% O(2). Oxygen tension had no effects on pS6, caveolin or LC3-II levels. IL-1-induced NO production was significantly reduced with decreased oxygen tensio...Continue Reading

References

Jul 17, 1975·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·I A Silver
May 1, 1994·Journal of Cellular Physiology·M Stefanovic-RacicC H Evans
Nov 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R L SmithD J Schurman
Oct 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·K FukudaS Tanaka
Jun 1, 1997·The EMBO Journal·H B JefferiesG Thomas
May 16, 1998·Current Biology : CB·R T Peterson, S L Schreiber
Jul 27, 1999·Osteoarthritis and Cartilage·M LotzK Kühn
Jul 27, 1999·Osteoarthritis and Cartilage·J PelletierJ Martel-Pelletier
Aug 24, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B FermorF Guilak
Feb 13, 2002·Arthritis and Rheumatism·Marcello Del Carlo, Richard F Loeser
Jul 29, 2003·Aging Cell·Jo S PriceIan M Clark
Sep 18, 2003·Osteoarthritis and Cartilage·Y E HenrotinJ-P L Pujol
Apr 9, 2004·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·James A MartinJoseph A Buckwalter
Jul 20, 2004·Apoptosis : an International Journal on Programmed Cell Death·H I RoachJ B Kouri
Feb 18, 2006·Molecular Cell·Liping LiuM Celeste Simon
Apr 14, 2006·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·K HashimotoC Hamanishi
Nov 1, 2006·Arthritis and Rheumatism·P I MilnerJ S Gibson
Nov 21, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ralph V Shohet, Joseph A Garcia
Dec 8, 2007·The Biochemical Journal·Cormac T Taylor
Aug 23, 2008·Journal of Cellular Physiology·Antonia F ChenBeverley Fermor
Nov 1, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Siriporn PeansukmaneeAli Mobasheri
Apr 3, 2009·Genes & Development·Eileen White, Scott W Lowe
Jun 13, 2009·Arthritis Research & Therapy·Steven B Abramson, Mukundan Attur
Jan 22, 2010·Apoptosis : an International Journal on Programmed Cell Death·M Almonte-BecerrilJ B Kouri

❮ Previous
Next ❯

Citations

Sep 29, 2011·Nature Reviews. Rheumatology·Andrew A Pitsillides, Frank Beier
Feb 7, 2014·Rheumatology·Chao ShenXiao-Dong Chen
Jun 7, 2014·Tissue Engineering. Part a·Tim W G M SpittersMarcel Karperien
Jan 22, 2013·Mitochondrion·María J López-ArmadaMarta N Valcárcel-Ares
Jan 22, 2013·Arthritis and Rheumatism·Brendan L ThomsChristopher L Murphy
Oct 11, 2015·Joint, Bone, Spine : Revue Du Rhumatisme·Yu-Sheng LiGuang-Hua Lei
Mar 29, 2011·Clinical and Experimental Immunology·C Ní CheallaighJ Harris
Apr 4, 2021·International Journal of Molecular Sciences·Maria Teresa ValentiMonica Mottes

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autophagy Networks

Autophagy is a lysosomal pathway that involves degradation of proteins and functions in normal growth and pathological conditions, through a series of complex networks. The catabolic process involves delivery of proteins and organelles to the lysosome. Here is the latest research on autophagy networks.

Autophagy & Metabolism

Autophagy preserves the health of cells and tissues by replacing outdated and damaged cellular components with fresh ones. In starvation, it provides an internal source of nutrients for energy generation and, thus, survival. A powerful promoter of metabolic homeostasis at both the cellular and whole-animal level, autophagy prevents degenerative diseases. It does have a downside, however--cancer cells exploit it to survive in nutrient-poor tumors.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.