Hypoxia-specific, VEGF-expressing neural stem cell therapy for safe and effective treatment of neuropathic pain

Journal of Controlled Release : Official Journal of the Controlled Release Society
Hye-Lan LeeYoon Ha

Abstract

Vascular endothelial growth factor (VEGF) is an angiogenic cytokine that stimulates the differentiation and function of vascular endothelial cells. VEGF has been implicated in improving nervous system function after injury. However, uncontrolled overexpression of VEGF increases the risk of tumor formation at the site of gene delivery. For this reason, VEGF expression needs to be strictly controlled. The goal of the present study was to understand the effects of hypoxia-induced gene expression system to control VEGF gene expression in neural stem cells (NSCs) on the regeneration of neural tissue after sciatic nerve injury. In this study, we used the erythropoietin (Epo) enhancer-SV40 promoter system (EpoSV-VEGF-NSCs) for hypoxia-specific VEGF expression. We used three types of NSCs: DsRed-NSCs as controls, SV-VEGF-NSCs as uncontrolled VEGF overexpressing NSCs, and EpoSV-VEGF-NSCs. For comparison of VEGF expression at normoxia and hypoxia, we measured the amount of VEGF secreted. VEGF expression decreased at normoxia and increased at hypoxia for EpoSV-VEGF-NSCs; thus, EpoSV-VEGF-NSCs controlled VEGF expression, dependent upon oxygenation condition. To demonstrate the therapeutic effect of EpoSV-VEGF-NSCs, we transplanted each cel...Continue Reading

References

Jan 1, 1989·Plastic and Reconstructive Surgery·J R BainD A Hunter
Nov 1, 1986·Neuropathology and Applied Neurobiology·S E MackinnonD A Hunter
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G L Wang, G L Semenza
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P H MaxwellP J Ratcliffe
May 1, 1993·Journal of Neuropathology and Experimental Neurology·C SommerR R Myers
Jul 26, 1996·The Journal of Biological Chemistry·B H JiangG L Semenza
Apr 18, 1997·Brain Research. Developmental Brain Research·C N SvendsenT Ryken
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·K L JinD A Greenberg
Apr 20, 2004·Experimental Cell Research·P S WalmodE Bock
Oct 19, 2004·Neuropathology and Applied Neurobiology·A BrockingtonP J Shaw
Dec 21, 2005·Journal of Cellular and Molecular Medicine·Anne Marie ByrneJ H Harmey
Jan 4, 2006·Brain Research Reviews·Gila Moalem, David J Tracey
Mar 7, 2006·Current Drug Targets·Dale G Nagle, Yu-Dong Zhou
Apr 21, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bryan C Hains, Stephen G Waxman
Aug 5, 2006·Molecular Pharmacology·Qingdong Ke, Max Costa
Aug 25, 2007·Anesthesia and Analgesia·Michael A ThackerStephen B McMahon
Oct 2, 2007·Biochemical and Biophysical Research Communications·Sanga SadatEckhard Alt
May 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Shusheng WangEric N Olson
Sep 24, 2008·Trends in Biochemical Sciences·Mei Yee KohGarth Powis
Dec 20, 2008·Nature Reviews. Neuroscience·Erin D Milligan, Linda R Watkins
Jan 23, 2009·Current Opinion in Pharmacology·Silvia BenemeiPierangelo Geppetti
Sep 2, 2009·Molecular and Cellular Biology·Lluis MartorellJosé Martínez-González
Sep 11, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jose A Gomez-SanchezHugo Cabedo
Feb 26, 2010·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Sung Su AnYoon Ha
Jan 7, 2011·Neuropathology and Applied Neurobiology·F R Pereira LopesA M B Martinez
Jan 20, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sun Hwa KimDonghoon Choi
Jul 19, 2011·Pain·Troels S JensenRolf-Detlef Treede
Oct 23, 2012·Nature Neuroscience·Kira I MosherTony Wyss-Coray
Jun 26, 2013·British Journal of Anaesthesia·A Ellis, D L H Bennett
Jul 23, 2013·Nature Neuroscience·Veronique E MironCharles Ffrench-Constant
Sep 10, 2014·BioMed Research International·Silvia FranchiPaola Sacerdote

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Citations

Jun 12, 2018·BioMed Research International·Huixing WangKemei Shi
May 1, 2021·International Journal of Molecular Sciences·Hye-Lan LeeKeung-Nyun Kim

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