Hypoxia suppresses E-cadherin and enhances matrix metalloproteinase-2 expression favoring esophageal carcinoma migration and invasion via hypoxia inducible factor-1 alpha activation

Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus
S-w JingC-r Yang

Abstract

The goal of this study was to explore the molecular mechanism of hypoxia inducible factor-1 alpha (HIF-1 alpha) action on migration and invasion of esophageal carcinoma cells. We used cobalt chloride (CoCl(2) ) to mimic tumor hypoxic microenvironment and analyzed the expressions of E-cadherin, matrix metalloproteinase-2 (MMP-2), and HIF-1 alpha in esophageal carcinoma cells under hypoxia by reverse transcription polymerase chain reaction and Western blotting. To analyze the function of HIF-1 alpha in Eca109 and TE1 cells, we established stable HIF-1 alpha knockdown cells using small interfering RNA. Blocking effect was detected by Western blotting. The concentrations of MMP-2 protein in the conditioned medium were also determined by enzyme-linked immunosorbent assay. Wound-healing and cell invasion assay were used to evaluate the migration and invasion of esophageal carcinoma cells. After exposure to hypoxia, expressions of HIF-1 alpha protein in Eca109 and TE1 cells were upregulated, both mRNA and protein levels of E-cadherin were downregulated, and MMP-2 were upregulated (P < 0.05), whereas HIF-1 alpha mRNA had no significant change (P > 0.05). Small interfering RNA could block HIF-1 alpha effectively under hypoxia, then enha...Continue Reading

References

Jan 20, 1995·The Journal of Biological Chemistry·G L Wang, G L Semenza
Mar 21, 2002·Nature Reviews. Cancer·Adrian L Harris
May 3, 2003·Cancer Cell·Nicola J MabjeeshParaskevi Giannakakou
Sep 26, 2003·The American Journal of Pathology·Tsutomu ImaiIkuo Konishi
Sep 6, 2005·Biochemical and Biophysical Research Communications·Christopher J Schofield, Peter J Ratcliffe
May 18, 2007·Nature Reviews. Cancer·Héctor PeinadoAmparo Cano
May 27, 2008·Nature Reviews. Cancer·Mark W DewhirstBenjamin Moeller
Dec 30, 2008·Cancer Letters·Leigh EllisRoberto Pili
May 11, 2010·The Journal of Surgical Research·Heikki TakalaYlermi Soini
Dec 7, 2010·International Journal of Oncology·Shingo TsujinakaFumio Konishi
Jun 30, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Zuo-Lin XiangDong-Mei Gao

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Citations

May 9, 2015·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Luke Gammon, Ian C Mackenzie
Nov 22, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Katarzyna StarskaAnna Krześlak
May 13, 2015·Trends in Pharmacological Sciences·Jing Y Krzeszinski, Yihong Wan
Mar 7, 2015·Virchows Archiv : an International Journal of Pathology·Supriya SrivastavaManuel Salto-Tellez
Jun 4, 2013·Experimental Cell Research·Risa HaradaToshihiro Akisue
Jul 4, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shao Wu JingQing Xu
Sep 2, 2017·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·Z MaZ Yu
Feb 25, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Krishnan MohanKumarAkhil Maheshwari
Feb 6, 2016·International Journal of Oncology·Eiji IwataTakahide Komori
Jan 11, 2013·Journal of Cellular and Molecular Medicine·Murielle Mimeault, Surinder K Batra

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