Hypoxic Conditions in Crown Galls Induce Plant Anaerobic Responses That Support Tumor Proliferation

Frontiers in Plant Science
Lucy KerpenDaan A Weits

Abstract

Agrobacterium tumefaciens infection of wounded plant tissues causes the formation of crown gall tumors. Upon infection, genes encoded on the A. tumefaciens tumor inducing plasmid are integrated in the plant genome to induce the biosynthesis of auxin and cytokinin, leading to uncontrolled cell division. Additional sequences present on the bacterial T-DNA encode for opine biosynthesis genes, which induce the production of opines that act as a unique carbon and nitrogen source for Agrobacterium. Crown galls therefore become a very strong sink for photosynthate. Here we found that the increased metabolic demand in crown galls causes an increase in oxygen consumption rate, which leads to a steep drop in the internal oxygen concentration. Consistent with this, plant hypoxia-responsive genes were found to be significantly upregulated in crown galls compared to uninfected stem tissue. Following this observation, we aimed at understanding whether the low-oxygen response pathway, mediated by group VII ethylene response factor (ERF-VII) transcription factors, plays a role in the development of crown galls. We found that quintuple knock-out mutants of all ERF-VII members, which are incapable of inducing the hypoxic response, show reduced c...Continue Reading

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Citations

May 17, 2019·Nature·Daan A WeitsFrancesco Licausi
Oct 23, 2019·Journal of Integrative Plant Biology·Michael John HoldsworthAgata Zubrycka
Dec 20, 2019·The New Phytologist·Sjon HartmanLaurentius A C J Voesenek
Jan 17, 2020·The New Phytologist·Daan A WeitsFrancesco Licausi
Jun 18, 2020·Plants·Elena Loreti, Pierdomenico Perata
Aug 17, 2019·Frontiers in Plant Science·Thibault MeyerCéline Lavire
Mar 4, 2020·The New Phytologist·Maria Cristina ValeriElena Loreti
Dec 8, 2020·The New Phytologist·Rashmi SasidharanPierdomenico Perata
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Apr 2, 2021·Plant Physiology·Michael J Considine, Christine H Foyer
Jun 3, 2021·Plant Signaling & Behavior·Hu Tang, Huanhuan Liu
Mar 17, 2021·Plant & Cell Physiology·Daan Adriaan Weits

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Methods Mentioned

BETA
transgenic
microsensors
microsensor
transfection

Software Mentioned

SigmaPlot
BoxPlotR

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