Hypoxic preconditioning attenuates necroptotic neuronal death induced by global cerebral ischemia via Drp1-dependent signaling pathway mediated by CaMKIIα inactivation in adult rats

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Lixuan ZhanEn Xu

Abstract

Hypoxic preconditioning (HPC) alleviates the selective and delayed neuronal death in the hippocampal CA1 region induced by transient global cerebral ischemia (tGCI). This type of cell death may include different programmed cell death mechanisms, namely, apoptosis and necroptosis. Although apoptotic signaling is well defined, the mechanisms that underlie neuronal necroptosis are yet to be fully elucidated. In this study, we investigated whether HPC protects neurons from cerebral ischemia-induced necroptosis. We observed that tGCI up-regulated the expression of receptor-interacting protein (RIP) 3 and increased the interaction of RIP1-RIP3 in CA1 at the early stage of reperfusion. The pretreatment with HPC or necrostatin-1 decreased the expression of RIP3 and the formation of RIP1-RIP3 after tGCI. We also found that HPC decreased the expression and the activity of caspase-8 in CA1 after tGCI, and notably, the pretreatment with Z-VAD-FMK, a pan-caspase inhibitor, did not trigger necroptosis but attenuated the tGCI-induced neuronal damage. Furthermore, we demonstrated that HPC decreased the activation of calcium-calmodulin kinase (CaMK) IIα and the interaction of RIP1 and CaMKIIα induced by tGCI. Intriguingly, the pretreatment with...Continue Reading

References

May 1, 1979·Stroke; a Journal of Cerebral Circulation·W A Pulsinelli, J B Brierley
Jan 1, 1995·Annual Review of Physiology·A P Braun, H Schulman
Apr 5, 2002·The Biochemical Journal·Andy Hudmon, Howard Schulman
Dec 25, 2002·The Journal of Cell Biology·Mariusz KarbowskiRichard J Youle
Jan 13, 2006·Nature Chemical Biology·Alexei DegterevJunying Yuan
May 23, 2006·Nature Reviews. Neuroscience·Jeffrey M Gidday
Jan 16, 2007·Cardiovascular Research·Martin Vila-PetroffAlicia Mattiazzi
Jun 8, 2007·The Journal of Biological Chemistry·Chuang-Rung Chang, Craig Blackstone
Jun 26, 2007·Science·Tiago Fleming OuteiroAleksey G Kazantsev
Aug 8, 2007·Molecular and Cellular Biology·Marlène BrasSantos A Susin
Mar 4, 2008·Molecular Cell·Atsushi Tanaka, Richard J Youle
May 14, 2008·Current Molecular Medicine·Nele VanlangenakkerPeter Vandenabeele
Jun 24, 2008·Nature Reviews. Neuroscience·Andrew B KnottElla Bossy-Wetzel
Aug 13, 2008·The Journal of Cell Biology·Xiao-Jian HanMasayuki Matsushita
Aug 8, 2009·Annual Review of Genetics·Chunxin Wang, Richard J Youle
Apr 1, 2010·Science Signaling·Peter VandenabeeleTom Vanden Berghe
Jun 24, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Frances J NorthingtonLee J Martin
Dec 8, 2010·Journal of Neuroscience Methods·Bruno P MeloniNeville W Knuckey
Mar 18, 2011·Nature·Marcus E Peter
Oct 12, 2012·Nature·Mei-Ling A JoinerMark E Anderson
Oct 10, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A LauA M Jevnikar
Dec 29, 2013·Cancer Research·Motonari NomuraYasufumi Kaneda
Jan 24, 2014·Nature Reviews. Molecular Cell Biology·Tom Vanden BerghePeter Vandenabeele
Jul 22, 2014·Seminars in Cell & Developmental Biology·Jennifer V LuCraig M Walsh
Sep 6, 2014·Journal of Neuropathology and Experimental Neurology·Tingna ZhuEn Xu

❮ Previous
Next ❯

Citations

Apr 7, 2020·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Fang ShenGang Chen
Oct 18, 2020·Archives of Biochemistry and Biophysics·Suchan LiaoSiriporn C Chattipakorn
Oct 31, 2020·International Journal of Molecular Sciences·Shoma ArakiYasuo Watanabe
Mar 14, 2021·Translational Stroke Research·Xuejing ZhangJane Q Wang
Jul 1, 2021·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Yuefang LiXin Hu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.