Hypoxic preconditioning enhances cell hypoxia tolerance and correlated lncRNA and mRNA analysis

Life Sciences
Dong WangJunlin Zhou

Abstract

Hypoxia makes cells death but it is not always true. Short term anoxia can boost remaining cells activities and promote tissue repair. This study is to explore the effects of hypoxic preconditioning (HP) on osteoblasts and analyze the involved lncRNA and mRNA. Human osteoblast was used. Normoxic control group (N) were cultured in normoxic culture medium (L-DMEM, 10% FBS and 1% penicillin-streptomycin) 8 h. Long-term hypoxic group (H) were cultured in 4 h hypoxic culture medium (200 μmol/L CoCl2 and L-DMEM)/4 h normoxic culture medium. Hypoxic preconditioning group (HP) were received 3 times HP (cultured in 10 min hypoxic culture medium/10 min normoxic culture medium, which was HP 1times) and then cultured in 4 h hypoxic culture medium/4 h normoxic culture medium. Cell proliferation ability, osteonectin, transforming growth factor-β1, insulin like growth factors-1 and osteopontin were test. The lncRNA and mRNA was detected by high-throughput sequencing. p-Value < 0.05 was accepted as statistically significant. Cell proliferation activity: N > HP > P. Corrected osteonectin, TGF-β1, IGF-1 and osteopontin: HP > P > N. High-throughput sequencing of gene shown the expression of 4630 RNAs. Further, we identified that HP group made 239...Continue Reading

Citations

May 14, 2020·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Yan LiLeisi Tao
May 30, 2019·BioMed Research International·Yang LiuJunlin Zhou

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