Hypoxic preconditioning increases the protective effect of bone marrow mesenchymal stem cells on spinal cord ischemia/reperfusion injury

Molecular Medicine Reports
Zhilin WangHong Ma

Abstract

Transplantation of bone marrow mesenchymal stem cells (BMSCs) protect against spinal cord ischemia/reperfusion injury (SCIRI). However, a large number of transplanted BMSCs often undergo apoptosis, which severely affects the treatment outcome. Previous studies have demonstrated that hypoxic preconditioning effectively increases the survival rate of BMSCs following transplantation, and increases their protective effect on injured tissues. However, there have been few reports regarding roles of hypoxic preconditioning in SCIRI. The present study isolated rat BMSCs and separately transplanted hypoxia‑ and non‑hypoxia‑preconditioned BMSCs into the spinal cord tissues of rats with SCIRI. The role of hypoxic preconditioning in the promotion of the protective effect of BMSCs on SCIRI was investigated using neurological function scores, Evans blue staining, hematoxylin and eosin staining and terminal deoxynucleotidyl transferase dUTP nick end labeling. In addition, reverse transcription‑quantitative polymerase chain reaction and western blotting were used to detect the expression levels of hypoxia‑inducible factor 1α (HIF‑1α), and to investigate its possible underlying mechanism of action. The results indicated that hypoxic preconditio...Continue Reading

References

Jan 1, 1995·The Annals of Thoracic Surgery·M C MauneyC G Tribble
Aug 5, 2000·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·L Lang-LazdunskiM Lazdunski
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 7, 2003·The Annals of Thoracic Surgery·Yukio KuniyoshiYoshiyuki Nakasone
Apr 2, 2004·Neurobiology of Disease·Christos ProfyrisSteven Petratos
May 28, 2005·Seminars in Cardiothoracic and Vascular Anesthesia·Roderick G MacArthurScott A LeMaire
Apr 19, 2006·Journal of Cellular Biochemistry·Arnold I Caplan, James E Dennis
May 30, 2006·The Annals of Thoracic Surgery·Enyi ShiAbul Hasan Muhammad Bashar
Mar 27, 2007·The Annals of Thoracic Surgery·Enyi ShiAbul Hasan Muhammad Bashar
Feb 1, 2008·International Journal of Cardiology·Jian-an WangHan Chen
Jun 10, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Carolyn A CarrKieran Clarke
Aug 5, 2008·Current Pharmaceutical Design·Weihong Pan, Abba J Kastin
Jan 16, 2009·Journal of the American Society of Nephrology : JASN·Daqing MaMervyn Maze
Nov 19, 2009·Current Opinion in Anaesthesiology·Ashish C Sinha, Albert T Cheung
Sep 28, 2010·Biochemical and Biophysical Research Communications·Hongbao LiuXiaohui Tian
Oct 23, 2010·Molecular Cell·Amar J MajmundarM Celeste Simon
Nov 11, 2010·Life Sciences·Karen M PetersonMartin Rodriguez-Porcel
Feb 3, 2012·Cellular and Molecular Neurobiology·Neetu SinghVikas Mishra

❮ Previous
Next ❯

Citations

Jul 28, 2016·Stem Cells Translational Medicine·Chengcheng CuiJieli Chen
Jul 18, 2017·Neurological Research·Yong QiaoXiuwen Wang
Jan 27, 2017·Neural Regeneration Research·Jian-An LiXiao-Yu Yang
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ruixi LuoYounan Chen
Aug 13, 2021·Stem Cell Research & Therapy·Qingyin LinYanzhou Yang

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence
PCR
density gradient centrifugation
flow cytometry
fluorescence microscopy

Software Mentioned

GraphPad
Graphpad Prism
Image J

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis