PMID: 11902755Mar 21, 2002Paper

Hysteroscopic insemination of low numbers of flow sorted fresh and frozen/thawed stallion spermatozoa

Equine Veterinary Journal
A C LindseyE L Squires

Abstract

The objective of this experiment was to determine the effects of flow cytometric sorting and freezing on stallion sperm fertility. A 2 x 2 factorial design was used to delineate effects of flow sorting and freezing spermatozoa. Oestrus was synchronised (July-August) in 41 mares by administering 10 ml altrenogest (2.2 mg/ml) per os for 10 consecutive days, followed by 250 microg cloprostenol i.m. on Day 11. Ovulation was induced by administering 3,000 iu hCG i.v. either 6 h (fresh spermatozoa) or 30 h (frozen/thawed spermatozoa) prior to insemination. Mares were assigned randomly to one of 4 sperm treatment groups. Semen was collected from 2 stallions with an artificial vagina and processed for each treatment. Treatment 1 (n = 10 mare cycles) consisted of fresh, nonsorted spermatozoa and Treatment 2 (n = 16 mare cycles) of fresh, flow sorted spermatozoa. Spermatozoa to be sorted were stained with Hoechst 33342 and sorted into X- and Y-chromosome-bearing populations based on DNA content using an SX MoFlo sperm sorter. Treatment 3 (n = 16 mare cycles) consisted of frozen/thawed nonsorted spermatozoa (frozen at 33.5 x 106 sperm/ml in 0.25 ml straws) and Treatment 4 (n = 15 mare cycles) of flow sorted frozen/thawed spermatozoa (froz...Continue Reading

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Citations

Oct 13, 2006·Reproduction, Fertility, and Development·Rebecca E SpindlerDavid E Wildt
Apr 23, 2014·Reproduction in Domestic Animals = Zuchthygiene·J L J GovaereA de Kruif
Aug 31, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Roberto PuglisiRiccardo Aleandri
Jul 25, 2008·Reproduction in Domestic Animals = Zuchthygiene·D Rath, L A Johnson
Jul 28, 2006·Reproduction in Domestic Animals = Zuchthygiene·J Aurich, C Aurich
Aug 14, 2002·Reproduction in Domestic Animals = Zuchthygiene·L H A Morris, W R Allen
Mar 21, 2002·Equine Veterinary Journal·T Katila
May 7, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·F K HollinsheadJ K O'Brien
Jul 13, 2005·Reproduction in Domestic Animals = Zuchthygiene·W R Allen
Dec 19, 2008·Reproduction in Domestic Animals = Zuchthygiene·G MariM Spinaci
Nov 3, 2007·Reproduction in Domestic Animals = Zuchthygiene·S P de GraafJ K O'Brien
Dec 7, 2006·Reproduction, Fertility, and Development·S P de GraafJ K O'Brien
Jul 25, 2019·Animals : an Open Access Journal From MDPI·Alicja KowalczykMarian Kuczaj
Feb 27, 2004·Reproduction, Fertility, and Development·J K O'BrienW M C Maxwell
Nov 16, 2019·Reproduction, Fertility, and Development·E L Squires

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