PMID: 11905434Mar 22, 2002Paper

Hysteroscopic insemination of mares with low numbers of nonsorted or flow sorted spermatozoa

Equine Veterinary Journal
A C LindseyJ E Bruemmer

Abstract

The objectives of this study were 1) to compare pregnancy rates resulting from 2 methods of insemination using low sperm numbers and 2) to compare pregnancy rates resulting from hysteroscopic insemination of 5 x 106 nonsorted and 5 x 106 spermatozoa sorted for X- and Y-chromosome-bearing populations (flow sorted). Semen was collected with an artificial vagina from 2 stallions of known acceptable fertility. Oestrus was synchronised (June to July) in 40 mares, age 3-10 years, by administering 10 ml altrenogest orally for 10 consecutive days, followed by 250 microg cloprostenol i.m. on Day 11. All mares were given 3000 iu hCG i.v. at the time of insemination to induce ovulation. Mares were assigned randomly to 1 of 3 treatment groups: mares in Treatment 1 (n = 10) were inseminated with 5 x 10(6) spermatozoa deposited deep into the uterine horn with the aid of ultrasonography. Mares in Treatment 2 (n = 10) were inseminated with 5 x 10(6) spermatozoa deposited onto the uterotubal junction papilla via hysteroscopic insemination. Mares in Treatment 3 (n = 20) were inseminated using the hysteroscopic technique with 5 x 10(6) flow sorted spermatozoa. Spermatozoa were stained with Hoechst 33342 and sorted into X- and Y-chromosome-bearing...Continue Reading

References

Jul 1, 1992·Equine Veterinary Journal·V Bracher, W R Allen
Jun 1, 1988·Biology of Reproduction·J J ParrishN L First
Mar 29, 2000·Theriogenology·G E SeidelD G Cran
Jun 1, 2000·Theriogenology·B R BuchananE L Squires

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Citations

Jun 9, 2004·Reproduction in Domestic Animals = Zuchthygiene·S VerberckmoesA de Kruif
Jul 13, 2005·Reproduction in Domestic Animals = Zuchthygiene·W R Allen
Apr 14, 2007·Journal of Dairy Science·I CerchiaroL Gallo
Dec 19, 2008·Reproduction in Domestic Animals = Zuchthygiene·G MariM Spinaci
Feb 27, 2004·Reproduction, Fertility, and Development·J K O'BrienW M C Maxwell
Nov 14, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·D RathS Washausen
Aug 14, 2002·Reproduction in Domestic Animals = Zuchthygiene·L H A Morris, W R Allen
Jul 25, 2008·Reproduction in Domestic Animals = Zuchthygiene·D Rath, L A Johnson
Nov 26, 2015·Reproduction in Domestic Animals = Zuchthygiene·C M Balao da SilvaF J Peña
Mar 21, 2002·Equine Veterinary Journal·T Katila
Jan 12, 2007·Reproduction in Domestic Animals = Zuchthygiene·P KlincD Rath

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