Hyul-Tong-Ryung suppresses PMA-induced MMP-9 expression by inhibiting AP-1-mediated gene expression via ERK 1/2 signaling pathway in MCF-7 human breast cancer cells

Immunopharmacology and Immunotoxicology
Kyoung-Sook KimJai-Heon Lee

Abstract

Our previous study has demonstrated that the methanol extract of Hyul-Tong-Ryung (HM) specifically suppresses the phorbol 12-myristate 13-acetate (PMA)-induced matrix metalloproteinase-9 (MMP-9) production through the inhibition of MMP-9 mRNA expression in MCF-7 human breast carcinoma cells. However, the molecular mechanisms involved in transcriptional suppression of MMP-9 by HM in PMA-induced MCF-7 cells are not known. In this study, we aimed to elucidate the molecular mechanisms involved in the inhibition of MMP-9 expression by HM in PMA-induced MCF-7 cells. The results of promoter assay and EMSA showed that HM specifically inhibits MMP-9 gene expression by blocking PMA-stimulated activation of activator protein-1 (AP-1). In addition, PMA-stimulated phosphorylation of extracellular signal regulated kinase 1/2 (ERK 1/2) was suppressed by HM treatment, whereas the phosphorylation of either c-Jun N-terminal kinase (JNK) or p38 mitogen-activated protein kinase (MAPK) was not affected. HM could inhibit the PMA-induced MMP-9 expression through suppression of the transcriptional activity of MMP-9 gene in MCF-7 cells. These results indicate that HM inhibits PMA-induced MMP-9 expression by blocking the activation of activator protein-...Continue Reading

References

Jun 13, 2001·Oncogene·E Shaulian, M Karin
May 7, 2002·Nature Reviews. Cancer·Mikala Egeblad, Zena Werb
Feb 11, 2003·Journal of Ethnopharmacology·Byung-Yoon ChaCheorl-Ho Kim
Jul 8, 2003·The Journal of Pathology·Ivan Stamenkovic
Nov 19, 2003·Molecular and Cellular Biochemistry·Sajal ChakrabortiTapati Chakraborti
Nov 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tae-Wook ChungCheorl-Ho Kim
May 24, 2005·Biochimica Et Biophysica Acta·Mikael Björklund, Erkki Koivunen

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