I-motif structures formed in the human c-MYC promoter are highly dynamic--insights into sequence redundancy and I-motif stability.

PloS One
Jixun DaiDanzhou Yang

Abstract

The GC-rich nuclease hypersensitivity element III1 (NHE III1) of the c-MYC promoter largely controls the transcriptional activity of the c-MYC oncogene. The C-rich strand in this region can form I-motif DNA secondary structures. We determined the folding pattern of the major I-motif formed in the NHE III1, which can be formed at near-neutral pH. While we find that the I-motif formed in the four 3' consecutive runs of cytosines appears to be the most favored, our results demonstrate that the C-rich strand of the c-MYC NHE III1 exhibits a high degree of dynamic equilibration. Using a trisubstituted oligomer of this region, we determined the formation of two equilibrating loop isomers, one of which contains a flipped-out cytosine. Our results indicate that the intercalative cytosine+-cytosine base pairs are not always necessary for an intramolecular I-motif. The dynamic character of the c-MYC I-motif is intrinsic to the NHE III1 sequence and appears to provide stability to the c-MYC I-motif.

References

Jan 1, 1992·Annual Review of Biochemistry·K B MarcuA J Patel
Jan 1, 1991·Advances in Cancer Research·C A Spencer, M Groudine
Jan 1, 1990·Advances in Cancer Research·I Magrath
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R TaubP Leder
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R Dalla-FaveraC M Croce
Apr 21, 1995·The Journal of Biological Chemistry·E F MichelottiD Levens
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Tomonaga, D Levens
Oct 18, 1996·Cell·K W Kinzler, B Vogelstein
Apr 4, 1998·Nucleic Acids Research·T SimonssonM Kubista
Jul 17, 1998·The American Journal of Pathology·N N NupponenT Visakorpi
Dec 22, 1998·Molecular and Cellular Biology·C V Dang
Mar 20, 1999·The Journal of Investigative Dermatology·H Schlagbauer-WadlB Jansen
Jun 23, 1999·Oncogene·C E NesbitE V Prochownik
Feb 19, 2000·Current Opinion in Genetics & Development·S PelengarisT Littlewood
Jun 14, 2000·Current Opinion in Structural Biology·M Guéron, J L Leroy
Nov 10, 2000·Biochemical and Biophysical Research Communications·T SimonssonM Vorlickova
Aug 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Adam Siddiqui-JainLaurence H Hurley
Jan 31, 2003·Genes, Chromosomes & Cancer·Charles P HarrisPulivarthi H Rao
Sep 23, 2003·Expert Opinion on Therapeutic Targets·Stella Pelengaris, Mike Khan
Oct 1, 2003·Journal of Molecular Biology·Jean Louis Leroy
Jul 15, 2004·Journal of the American Chemical Society·Jeyaprakashnarayanan SeenisamyLaurence H Hurley
Jul 15, 2004·Journal of the American Chemical Society·Anh Tuân PhanDinshaw J Patel
Aug 12, 2006·Nucleosides, Nucleotides & Nucleic Acids·Danzhou Yang, Laurence H Hurley
Mar 10, 2007·Journal of Medicinal Chemistry·Tian-Miao OuLian-Quan Gu
May 9, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Fedor Kouzine, David Levens
Jan 15, 2008·Nature Structural & Molecular Biology·Fedor KouzineDavid Levens
Feb 6, 2008·Nature Structural & Molecular Biology·Christophe Lavelle

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Citations

Apr 18, 2014·The Journal of Physical Chemistry. B·Sung Eun KimSeok-Cheol Hong
Aug 22, 2014·Nucleic Acids Research·Eric Largy, Jean-Louis Mergny
Oct 18, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jingjing CuiEdwin A Lewis
Aug 26, 2015·Journal of the American Chemical Society·Irina V NesterovaEvgueni E Nesterov
Dec 23, 2016·Scientific Reports·Elisé P WrightZoë A E Waller
Mar 16, 2017·Journal of the American Chemical Society·Aaron M FlemingCynthia J Burrows
Oct 8, 2013·Physical Chemistry Chemical Physics : PCCP·Hou U UngThomas Hellman Morton
Dec 7, 2018·Chemical & Pharmaceutical Bulletin·Shadi Sedghi Masoud, Kazuo Nagasawa
Dec 29, 2018·BMC Genomics·Mauno Vihinen
Nov 30, 2019·Nucleic Acids Research·Elisé P WrightZoë A E Waller
Nov 19, 2011·Angewandte Chemie·Anna Lena LiebleinHarald Schwalbe
Jun 25, 2013·Chembiochem : a European Journal of Chemical Biology·Anna Lena LiebleinHarald Schwalbe
Nov 23, 2017·Chemical Science·Manish DebnathJyotirmayee Dash
Mar 18, 2020·Chemical Science·Puja SahaJyotirmayee Dash
Nov 3, 2017·Chemistry : a European Journal·Hala Abou AssiMasad J Damha
Aug 21, 2018·Nucleic Acids Research·Hala Abou AssiMasad J Damha
Jan 31, 2021·Pharmaceuticals·Susie L Brown, Samantha Kendrick
Sep 30, 2019·NAR Genomics and Bioinformatics·Efres Belmonte-Reche, Juan Carlos Morales
Apr 24, 2021·Scientific Reports·Minami Hasegawa-Ogawa, Hirotaka James Okano
May 12, 2021·Journal of Natural Products·Alexander S TikhomirovAndrey E Shchekotikhin
Jul 18, 2021·Life Science Alliance·Bidyut K MohantyPhilip H Howe
Jan 23, 2018·Journal of the American Chemical Society·Lutan LiuTigran V Chalikian
Aug 29, 2019·ACS Omega·Michael M MolnarRandy M Wadkins
Sep 3, 2011·Journal of the American Chemical Society·Jungkweon ChoiTetsuro Majima
Sep 2, 2021·Nucleic Acids Research·Jeong Hwan HurKyeong Kyu Kim

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