PMID: 9159115May 27, 1997Paper

I2B is a small cytosolic protein that participates in vacuole fusion

Proceedings of the National Academy of Sciences of the United States of America
P SlusarewiczW Wickner

Abstract

Saccharomyces cerevisiae vacuole inheritance requires two low molecular weight activities, LMA1 and LMA2. LMA1 is a heterodimer of thioredoxin and protease B inhibitor 2 (I2B). Here we show that the second low molecular weight activity (LMA2) is monomeric I2B. Though LMA2/I2B was initially identified as a protease B inhibitor, this protease inhibitor activity is not related to its ability to promote vacuole fusion: (i) Low Mr protease B inhibitors cannot substitute for LMA1 or LMA 2, (ii) LMA1 and LMA2 promote the fusion of vacuoles from a strain that has no protease B, (iii) low concentrations of LMA2 that fully inhibit protease B do not promote vacuole fusion, and (iv) LMA1, in which I2B is complexed with thioredoxin, is far more active than LMA2/I2B in promoting vacuole fusion and far less active in inhibiting protease B. These studies establish a new function for I2B.

References

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Citations

Nov 8, 2007·PloS One·Michela Felberbaum-CortiJean Gruenberg
Feb 24, 2001·Antioxidants & Redox Signaling·T D Lockwood
Aug 15, 2002·The Journal of Cell Biology·Gary EitzenWilliam Wickner
Jul 9, 2004·The EMBO Journal·Naomi ThorngrenAlexey J Merz
Aug 31, 2000·Annual Review of Biochemistry·W Wickner, A Haas
Nov 3, 2006·Journal of Molecular Biology·Michael A WhiteMark E Dumont
Oct 16, 2004·The Journal of Biological Chemistry·Youngsoo JunWilliam Wickner
Mar 2, 2005·The Journal of Biological Chemistry·Vincent J StaraiWilliam Wickner
Mar 10, 2007·The Journal of Biological Chemistry·Rutilio A Fratti, William Wickner
Feb 23, 2017·The Journal of Biological Chemistry·Mathias HohlAndreas Schaller
Mar 28, 2006·The Journal of Biological Chemistry·Bridget L Decker, William T Wickner

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