PMID: 6403654Apr 1, 1983Paper

Ia expression by vascular endothelium is inducible by activated T cells and by human gamma interferon

The Journal of Experimental Medicine
J S PoberK A Ault

Abstract

We have used monoclonal antibody binding, measured by radioimmunoassay, fluorescence flow cytometry, and ultrastructural immunocytochemistry, to measure expression of Ia antigens on cultured human umbilical vein endothelial (HUVE) cells. Under standard culture conditions, HUVE cells do not express Ia antigens. However, treatment of primary HUVE cultures with phytohemagglutinin induces the expression of Ia antigens. Every endothelial cell in the culture becomes Ia-positive and endothelial cells appear to synthesize Ia. HLA-A,B is concomitantly increased. The expression of Ia appears to be mediated by T cells because (a) pretreatment of primary HUVE cultures with OKT3 plus complement blocks the action of the lectins but not of medium conditioned by lectin-activated peripheral blood mononuclear cells; (b) co-culture of endothelial cells with allogeneic T cells, in the absence of lectin, also induces endothelial Ia; and (c) human immune (gamma) interferon, produced by Chinese hamster ovary cells transfected with the human gamma interferon gene, directly induces endothelial Ia. During co-culture with lymphocytes, about one-third of the endothelial cells are Ia-positive after 24 h and all of the endothelial cells are Ia-positive by 7...Continue Reading

References

Jun 1, 1975·Transplantation·H HirschbergE Thorsby
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E L ReinherzS F Schlossman
May 1, 1977·European Journal of Immunology·K I WelshJ R Batchelor
Aug 1, 1977·The Journal of Clinical Investigation·J R Moraes, P Stastny
May 2, 1980·Science·D Brouty-Boyé, B R Zetter
Nov 1, 1980·The Journal of Experimental Medicine·R M SteinmanI S Mellman
Dec 1, 1980·The Journal of Experimental Medicine·P S SteegJ J Oppenheim
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J S Pober, M A Gimbrone
Aug 1, 1982·The Journal of Cell Biology·G F Schreiner, R S Cotran
Dec 1, 1980·The Journal of Experimental Medicine·M G ScherE R Unanue
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D A ShackelfordJ L Strominger

❮ Previous
Next ❯

Citations

Feb 1, 1990·Annals of Neurology·A NoronhaM A Jensen
Feb 1, 1990·Journal of Cellular Physiology·R M TuderK G Bensch
Mar 1, 1990·Journal of Cellular Physiology·Y SaegusaD Cavender
Jan 1, 1991·Rheumatology International·A D Blann, D G Scott
Jan 1, 1994·Rheumatology International·R SauraK Mizuno
Jan 1, 1990·Archives of Dermatological Research·A ReanoJ Viac
Jan 1, 1985·Journal of Cancer Research and Clinical Oncology·B KochJ R Kalden
Jan 1, 1993·Breast Cancer Research and Treatment·M J Kennedy, R J Jones
Jan 1, 1987·Acta Neuropathologica·M K Axthelm, S Krakowka
Mar 1, 1993·Digestive Diseases and Sciences·T R StevensD S Rampton
Jul 16, 1984·Klinische Wochenschrift·C MüllerH D Waller
Sep 24, 1999·Immunologic Research·J S Pober
Jan 1, 1986·Immunologic Research·J J FungR J Duquesnoy
Jan 1, 1991·Immunologic Research·D R Johnson, J S Pober
Jul 1, 1984·Cellular Immunology·J J Donnelly, R A Prendergast
Jul 1, 1985·Journal of the Neurological Sciences·U Traugott, C S Raine
Jan 1, 1987·Molecular Aspects of Medicine·P Libby
Dec 1, 1985·Molecular Immunology·L GrafV Schirrmacher
Apr 1, 1994·Pharmacology & Therapeutics·T C Johnson
Jul 1, 1986·Journal of Reproductive Immunology·J R Head, S D Gaede
Mar 1, 1988·Immunology Letters·R M Steinman
Dec 1, 1988·Journal of Neuroimmunology·C S Raine, L C Scheinberg
Sep 1, 1995·Journal of Neuroimmunology·Y WangJ Greenwood
Sep 1, 1992·Journal of Virological Methods·E W AdesF Candal
Jan 1, 1987·Immunology Today·G C de GastE Gluckman
Nov 1, 1989·Immunology Today·A Mantovani, E Dejana
Oct 1, 1993·Immunology Today·F GarridoP L Stern

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.