Abstract
Autophagy serves a critical function in the pathogenesis, response to therapy and clinical outcome in cancers. Although a recent report showed a role of iASPP in suppressing autophagy, its potential activity as a regulator of autophagy has not been investigated in lung cancer. Here we investigated the potential function and molecular mechanism of iASPP in mediating autophagy in human non-small-cell lung cancer. Our data suggested that forced expression of iASPP triggered autophagic flux, while inhibition of iASPP suppressed autophagy at the autophagsome formation stage in vitro. Furthermore, in vivo overexpression of iASPP in SCID/NOD mice promoted tumorigenesis and autophagy, with an increase in the conversion from LC3-I to LC3-II. The effects of iASPP were mediated through activation of mTOR pathway. Finally, cytoplasmic iASPP expression was upregulated in lung cancer patients, and was identified as an independent poor prognostic factor for lung cancer-specific death in patient samples. Taken together, our data showed that iASPP could promote tumor growth by increasing autophagic flux, and iASPP could serve as a poor prognostic factor and a potential therapeutic target in lung cancer.
References
Jan 14, 2003·Nature Genetics·Daniele BergamaschiXin Lu
Jun 16, 2007·Experimental Cell Research·Magdalena J LaskaBjørn A Nexø
Feb 16, 2008·Structure·Ross Alexander RobinsonChristian Siebold
Sep 18, 2008·Cancer Investigation·Ze-Jun LiuJie Chen
Mar 10, 2009·Cell Cycle·Boyi Gan, Ronald A DePinho
Mar 21, 2009·Leukemia Research·Hang LiuJianxiang Wang
Mar 2, 2010·Cancer Biotherapy & Radiopharmaceuticals·Jing-Hua RenGang Wu
Aug 31, 2010·Chinese Journal of Cancer·Ming-Shu PangYa-Jun Guo
Jan 5, 2011·BMC Cancer·Jinfeng ChenWen G Jiang
Jun 1, 2011·Prostate Cancer and Prostatic Diseases·B ZhangL H Cai
Sep 20, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lili JiangAnnie N Y Cheung
Jan 13, 2012·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Apr 28, 2012·Apoptosis : an International Journal on Programmed Cell Death·Yun CaiZe-Jun Liu
Jul 25, 2013·Cancer Letters·Yu-Jiao SongLi-Xin Wei
Apr 30, 2014·Journal of Cell Science·Anissa ChikhDaniele Bergamaschi
Aug 26, 2014·Annals of Surgical Oncology·Ji Won KimSang Yoon Kim
Oct 24, 2014·Cell Death & Disease·E V MorrisR J Bryant
Mar 4, 2015·Current Topics in Medicinal Chemistry·Shan LiRong Sheng
Mar 10, 2015·Cancer Letters·Hao-Zheng YangHan-Bing Zou
Apr 22, 2015·Oncogene·V W Rebecca, R K Amaravadi
Aug 26, 2015·International Journal of Experimental Pathology·Pınar Aslan KoşarAna Beatriz Rodriguez Moratinos
Dec 17, 2015·Virulence·Ao ZhouShujun Zhang
Feb 28, 2016·Current Opinion in Pharmacology·Andrei S Chagin
Citations
Oct 9, 2018·Clinical Science·Jinfang BaoWeijie Yuan
Aug 21, 2018·The International Journal of Neuroscience·Mei SunHong Cheng
Feb 9, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Milan HanoZdena Sulová
Aug 10, 2019·Proceedings of the National Academy of Sciences of the United States of America·Shuo ChenXin Lu
Nov 28, 2019·Therapeutic Advances in Respiratory Disease·Shi-Xia LiaoYao Ou-Yang
Oct 17, 2019·BMC Cancer·Ka-Kui ChanAnnie Nga-Yin Cheung
Apr 18, 2021·Cellular and Molecular Life Sciences : CMLS·Xue LiHuaiyong Chen
Sep 22, 2021·Cell Biology International·Zijian WangShengjie Yang