IbeB is involved in the invasion and pathogenicity of avian pathogenic Escherichia coli

Veterinary Microbiology
Shaohui WangChengping Lu

Abstract

The ibeB gene in neonatal meningitis Escherichia coli (NMEC) contribute to the penetration of human brain microvascular endothelial cells (HBMECs). However, whether IbeB plays a role in avian pathogenic E. coli (APEC) infection remains unclear. Thus, this study was conducted to investigate the distribution of the ibeB gene in Chinese APEC strains and examine whether IbeB is involved in APEC pathogenicity. The ibeB gene was found in all 100 detected E. coli isolates with over 97% sequence homology. These results indicated that ibeB is a conserved E. coli gene irrelevant of pathotypes. To determine the role of ibeB in APEC pathogenicity, an ibeB mutant of strain DE205B was constructed and characterized. The inactivation of ibeB resulted in reduced invasion capacity towards DF-1 cells and defective virulence in animal models as compared to the wild-type strain. Animal infection experiments revealed that loss of ibeB decreased APEC colonization and invasion capacity in brains and lungs. These virulence-related phenotypes were partially recoverable by genetic complementation. Reduced expression levels of invasion- and adhesion-associated genes in ibeB mutant could be major reasons as evidenced by reduced ibeA and ompA expression. Th...Continue Reading

References

May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Mar 15, 1984·The New England Journal of Medicine·T SöderströmG Larson
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P DavanlooF W Studier
Feb 7, 1998·Veterinary Microbiology·S A PourbakhshJ M Fairbrother
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Sep 30, 2000·Applied and Environmental Microbiology·O ClermontE Bingen
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 29, 2002·The Journal of Infectious Diseases·James R Johnson, Thomas A Russo
Jun 19, 2003·Journal of Bacteriology·Sylvia FrankeDietrich H Nies
Oct 6, 2006·Journal of Clinical Microbiology·Maryvonne Moulin-SchouleurJorge Blanco
Mar 22, 2007·International Journal of Medical Microbiology : IJMM·Christa EwersLothar H Wieler

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Citations

Sep 26, 2013·Future Microbiology·Nadezhda GermanChristopher Rensing
Mar 12, 2016·BMC Microbiology·Livia PilattiWanderley Dias da Silveira

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