PMID: 7542569Mar 1, 1995Paper

IBMX induces calcium release from intracellular stores in rat sensory neurones

Cell Calcium
Y Usachev, A Verkhratsky

Abstract

The intracellular free calcium concentration ([Ca2+]i) was recorded from freshly isolated rat dorsal root ganglia (DRG) neurones by means of Fura-2 or Indo-1-based microfluorimetry. Extracellular application of IBMX at millimolar concentrations evoked transient elevations in [Ca2+]i. The amplitude and rate of rise of the [Ca2+]i transient increased with increasing IBMX concentration. The effects of IBMX on [Ca2+]i were not mimicked by direct manipulation of the intracellular level of cyclic nucleotides, nor incubation with dibutyryl-cAMP, cGMP or 8-bromo-cGMP; neither did treatment with forskolin induce similar [Ca2+]i transients. The IBMX-evoked [Ca2+]i elevation persisted in Ca(2+)-free extracellular solutions, suggesting its origination was from intracellular stores. Caffeine-induced depletion of internal Ca2+ pool prevented IBMX-evoked [Ca2+]i responses, and vice versa, IBMX treatment strongly reduced the amplitude of subsequent caffeine-induced [Ca2+]i elevation. Both ryanodine and procaine, agents known to interact with Ca(2+)-gated Ca2+ release channels of the intracellular endoplasmic reticulum calcium stores, effectively inhibited IBMX-induced [Ca2+]i transients. We suggest that, in addition to its known phosphodiester...Continue Reading

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Citations

Oct 24, 2012·Journal of Bioenergetics and Biomembranes·Nathan R PerronBärbel Rohrer
Jan 26, 1996·Neuroscience Letters·J A David, R M Pitman
Aug 2, 2002·European Journal of Pharmacology·Alexej Verkhratsky, Ole H Petersen
Jan 22, 2003·Journal of Neuroscience Methods·Natasha Solovyova, Alexei Verkhratsky
Jun 28, 2000·Journal of the Autonomic Nervous System·J W Daly
Jan 1, 1996·Life Sciences·S Kirischuk, A Verkhratsky
May 12, 2009·Biochimica Et Biophysica Acta·Kaipeng JingJong-Il Park
Jan 1, 1996·Cell Calcium·A Verkhratsky, A Shmigol
Mar 26, 1999·Cell Calcium·A J Verkhratsky, O H Petersen
Feb 13, 2010·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Adeola A SalawuAbayomi O Okanlawon
May 4, 2016·Cell and Tissue Research·Indrashis BhattacharyaSubeer S Majumdar
Jul 3, 1999·Physiological Reviews·A MeirR Rahamimoff
Jan 1, 1996·Cell Calcium·S KirischukA Verkhratsky

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