Ibuprofen causes photocleavage through ROS generation and intercalates with DNA: a combined biophysical and molecular docking approach

Physical Chemistry Chemical Physics : PCCP
Mohammed Amir HusainMohammad Tabish

Abstract

Ibuprofen is an important nonsteroidal anti-inflammatory drug endowed with various pharmacological and biological activities. In the present study, the photochemical properties of ibuprofen were evaluated by assaying the generation of various reactive oxygen species (ROS) such as superoxide, singlet oxygen and the hydroxyl radical. ROS generated by ibuprofen in the presence of white light causes DNA strand scission as observed by plasmid nicking assay. Ibuprofen induced ROS generation is also capable of causing DNA degradation in lymphocytes as observed by photocomet assay. ROS generation properties of ibuprofen were further strengthened by the formation of carbonyl groups in BSA and TBARS in linoleic acid as observed by carbonyl assay and lipid peroxidation assay respectively. We have also investigated the mode of interaction of ibuprofen with calf thymus DNA through a series of in vitro experiments. UV-visible spectroscopy established the formation of a complex between ibuprofen and Ct DNA. The steady state fluorescence experiments at different temperatures revealed a binding constant of ∼10(4) L mol(-1), which is indicative of intercalative binding between ibuprofen and the DNA helix. Analysis of the various thermodynamic pa...Continue Reading

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
Nov 1, 1991·Photochemistry and Photobiology·C S Foote
Aug 1, 1991·Photochemistry and Photobiology·T ArtusoN Paillous
Nov 1, 1987·Biochemical Pharmacology·G J Quinlan, J M Gutteridge
Jan 1, 1994·Free Radical Biology & Medicine·R RamanathanC H Tan
Nov 20, 1998·Journal of Photochemistry and Photobiology. B, Biology·L Martínez, C F Chignell
Dec 10, 1999·Biochemistry·J Ren, J B Chaires
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
Apr 5, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L MarrotC Agapakis-Causse
Aug 10, 2001·American Journal of Epidemiology·M TrevisanD Armstrong
Sep 27, 2002·Chembiochem : a European Journal of Chemical Biology·Giampietro ViolaHeiko Ihmels
May 21, 2003·Chemical Research in Toxicology·Giampietro ViolaArianna Barbafina
Aug 21, 2003·Environmental and Molecular Mutagenesis·Mark S RundellMichael J Plewa
Nov 4, 2004·Journal of Inorganic Biochemistry·Elene V Hackl, Yurij P Blagoi
Feb 25, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L MarrotJ R Meunier
Feb 24, 2007·Biomacromolecules·Alok ChakrabartyNitin Chattopadhyay
Aug 15, 2008·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mehrnaz Gharagozlou, Davar M Boghaei
Aug 30, 2008·Biomacromolecules·C N N'soukpoé-KossiH A Tajmir-Riahi
Oct 23, 2009·Photochemistry and Photobiology·Daniel H González MaglioJuliana Leoni
Jan 22, 2010·The Journal of Physical Chemistry. B·Dibakar SahooSankar Chakravorti
Jan 5, 2011·Biomacromolecules·E FroehlichH A Tajmir-Riahi
Aug 15, 2012·Journal of Cheminformatics·Marcus D HanwellGeoffrey R Hutchison
Feb 20, 2013·Journal of Photochemistry and Photobiology. B, Biology·Yoshiki SetoSatomi Onoue
Mar 13, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·G BracchittaP M Girard
Mar 28, 2013·Journal of Chemical Information and Modeling·Khaled M Elokely, Robert J Doerksen
Dec 5, 2013·The FEBS Journal·Mohammed A HusainMohammad Tabish
Jan 21, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Lina HanJian Shen
Apr 15, 2014·European Journal of Medicinal Chemistry·Rabindra Reddy PulimamidiHussain Shaik
Oct 26, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sayeed Ur RehmanMohammad Tabish
Dec 3, 2014·International Journal of Biological Macromolecules·Tarique SarwarMohammad Tabish
Dec 22, 2014·Archives of Biochemistry and Biophysics·Sayeed Ur RehmanMohammad Tabish

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Citations

Apr 17, 2016·Journal of Photochemistry and Photobiology. B, Biology·Mehtab ParveenP S Pereira Silva
May 9, 2015·Archives of Biochemistry and Biophysics·Sayeed Ur RehmanMohammad Tabish
Nov 12, 2016·European Journal of Medicinal Chemistry·Roya Esteghamat-PanahFatemeh Abyar
Nov 12, 2016·Nucleosides, Nucleotides & Nucleic Acids·Nahid Shahabadi, Farshad Shiri
Jun 19, 2017·Archives of Biochemistry and Biophysics·K M AbdullahImrana Naseem
Jun 27, 2018·Journal of Molecular Recognition : JMR·Khalid I Al QumaiziTasneem Fatma
May 14, 2020·Journal of Biomolecular Structure & Dynamics·Shahid Iqbal FarooqiMaham Yamin
Aug 23, 2017·Scientific Reports·Emmanuelle BignonAntonio Monari
Dec 6, 2016·Journal of Molecular Recognition : JMR·Razique AnwerTasneem Fatma
Dec 7, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Rajendra Prasad PaitandiDaya Shankar Pandey
Dec 9, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Arnab BhattacharyyaAkhil R Chakravarty
Jan 11, 2020·The Science of the Total Environment·Verónica Margarita Gutiérrez-NoyaRubi Romero
Dec 2, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Cosmin ButnarasuSonja Visentin
Apr 27, 2021·Journal of Biomolecular Structure & Dynamics·Sundararajan PonkarpagamKuppanagounder P Elango
Apr 30, 2021·European Journal of Medicinal Chemistry·Arnab BhattacharyyaAkhil R Chakravarty
Sep 29, 2017·Inorganic Chemistry·Rajendra Prasad PaitandiDaya Shankar Pandey

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