Ibuprofen mediates histone modification to diminish cancer cell stemness properties via a COX2-dependent manner.

British Journal of Cancer
Wenzhi ShenJianjun Li

Abstract

The anticancer potential of ibuprofen has created a broad interest to explore the clinical benefits of ibuprofen in cancer therapy. However, the current understanding of the molecular mechanisms involved in the anticancer potential of ibuprofen remains limited. Cancer stemness assays to validate ibuprofen function in vitro and in vivo. Histone modification assays to check the effect of ibuprofen on histone acetylation/methylation, as well as the activity of HDAC and KDM6A/B. Inhibitors' in vivo assays to evaluate therapeutic effects of various inhibitors' combination manners. In our in vitro studies, we report that ibuprofen diminishes cancer cell stemness properties that include reducing the ALDH + subpopulation, side population and sphere formation in three cancer types. In our in vivo studies, we report that ibuprofen decreases tumour growth, metastasis and prolongs survival. In addition, our results showed that ibuprofen inhibits inflammation-related stemness gene expression (especially ICAM3) identified by a high-throughput siRNA platform. In regard to the underlying molecular mechanism of action, we report that ibuprofen reduces HDACs and histone demethylase (KDM6A/B) expression that mediates histone acetylation and methy...Continue Reading

References

Nov 21, 2002·The Annals of Thoracic Surgery·Syed Suhail Ahmed QadriHenry Paul Redmond
Aug 20, 2004·Pharmacological Reviews·Daniel L SimmonsTimothy Hla
Dec 22, 2007·Nature Reviews. Molecular Cell Biology·D Leanne Jones, Amy J Wagers
Dec 8, 2009·Medicinal Research Reviews·Mohammed Naseer A Khan, Yong Sup Lee
Feb 16, 2011·Cell Research·Andrew J Bannister, Tony Kouzarides
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Mar 24, 2012·Cancer Cell·Jeffrey A MageeSean J Morrison
Apr 5, 2012·Nature Reviews. Genetics·Eric L Greer, Yang Shi
Jun 19, 2012·Cell Stem Cell·Jane E Visvader, Geoffrey J Lindeman
Jun 25, 2013·Nature Methods·Marina C PrewitzCarsten Werner
Jan 7, 2014·Biochimica Et Biophysica Acta·Chunbin Zou, Rama K Mallampalli
May 15, 2015·International Journal of Cancer. Journal International Du Cancer·Claudia BeckerChristoph R Meier
Jun 26, 2015·Journal of the National Cancer Institute·Driss Ait OuakrimAung Ko Win
Jul 23, 2015·Revista brasileira de ginecologia e obstetrícia : revista da Federação Brasileira das Sociedades de Ginecologia e Obstetrícia·Renilton Aires LimaAgnaldo Lopes da Silva Filho
May 20, 2016·Annual Review of Pathology·Dany Nassar, Cédric Blanpain
Dec 14, 2017·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Laura P StabileJill M Siegfried
Dec 14, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Poulami MandalSuman Mukhopadhyay
Jul 22, 2016·Signal Transduction and Targeted Therapy·Wenzhi ShenRong Xiang
Feb 8, 2018·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Osama DandahDiana Anderson
Feb 27, 2018·Cancer Letters·Wenzhi ShenYunping Luo
May 8, 2018·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Wenzhi ShenShuangtao Zhao
Jul 18, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Dehong LuoWenzhi Shen

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Citations

Oct 7, 2021·Environmental Science and Pollution Research International·Smita KumariRashmi K Ambasta

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Methods Mentioned

BETA
acetylation
PCR
immunoprecipitation
Assay
pull-down
histone acetylation

Software Mentioned

Flowjo
GraphPad
GraphPad Prism5

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