ICAM-1 expression in vaginal cells as a potential biomarker for inflammatory response

Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Hyewon YounChi H Lee

Abstract

This study aimed to elucidate the mechanisms that may lead to an efficient strategy to induce a suitable host response of the vaginal mucosa upon exposure to intravaginally delivered exogenous compounds. It was hypothesized that the upregulation of intercellular adhesion molecule (ICAM)-1 gene expression may reflect the inflammatory response evoked by exogenous compounds. Major emphasis was placed on ethylenediamine tetraacetic acid (EDTA) which was added as a synergistic agent to conventional spermicidal agents or anti-HIV drugs. The levels of ICAM-1 mRNA were examined as a surrogate marker for inflammatory response in human vaginal epithelial cells upon exposure to EDTA or interleukin (IL)-1beta (i.e. positive control, 25 mM). The effects of estrogen on EDTA-induced ICAM-1 expression were also evaluated for the estrogen involvement in the inflammatory process of the vaginal mucosa. ICAM-1 expression in human vaginal cells (VK2/E6E7 cells) increased as EDTA concentration added to human vaginal cell lines increased. The effects of estrogen on EDTA-induced ICAM-1 expression in human vaginal epithelial cells were estrogen-concentration dependent; estrogen at lower concentrations (approximately 1-10 nM) did not affect ICAM-1 expre...Continue Reading

References

Sep 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·T E WieseS C Brooks
Aug 1, 1988·Journal of Virological Methods·R PauwelsE De Clercq
Sep 1, 1983·Molecular and Cellular Biology·J W BodnarD C Ward
Jun 1, 1996·Journal of Pharmaceutical Sciences·C H LeeY W Chien
Aug 1, 1996·Cell Calcium·M D Bootman
Jun 5, 1998·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·C J KirkpatrickM Otto
Dec 30, 1999·Journal of Leukocyte Biology·K A Roebuck, A Finnegan
Nov 7, 2002·Climacteric : the Journal of the International Menopause Society·H P G Schneider, UNKNOWN Executive Committee of the IMS
Apr 1, 2003·International Journal of STD & AIDS·Devon D BrewerFrançois Vachon
May 3, 2003·The Journal of Clinical Endocrinology and Metabolism·Nicola ColacurciGiuseppe Paolisso
Oct 17, 2003·The Journal of Pharmacology and Experimental Therapeutics·Haisong JuRobert N Willette
Oct 18, 2003·Virchows Archiv : an International Journal of Pathology·Ben VainerThomas Horn
Oct 24, 2003·Neurobiology of Disease·Michelle MaldonadoPeter B Crino
Jun 16, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H ShirasakiT Himi
Apr 15, 2005·Molecular Immunology·Andrew G Bowie, Ismar R Haga
Jun 14, 2005·Trends in Endocrinology and Metabolism : TEM·Bukhtiar H Shah
Jul 27, 2005·Teaching and Learning in Medicine·Halit Hami Oz
Jul 18, 2006·Diabetic Medicine : a Journal of the British Diabetic Association·D W BowdenD M Herrington
Aug 5, 2006·International Journal of Pharmaceutics·Sravanthi CheetiChi H Lee

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Jul 24, 2015·AIDS Research and Human Retroviruses·Andrea Ries ThurmanGustavo Doncel
Jun 9, 2016·PloS One·Jamal QualaiMeritxell Genescà
Dec 9, 2014·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Igor TsaurRoman A Blaheta

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