ICAM-1 induction in respiratory cells exposed to a replication-deficient recombinant adenovirus in vitro and in vivo

Gene Therapy
Elena NicolisGiulio Cabrini

Abstract

Administration of replication-deficient recombinant adenoviruses (Ad) designed as vectors for gene transfer to the airway tract of rats and monkeys has been associated with a dose-dependent inflammatory process a few days after viral exposure. Among the cellular mechanisms possibly involved, we investigated the expression of intercellular adhesion molecule-1 (ICAM-1), which is known to be induced by parainfluenza, adenovirus type 5 and respiratory syncytial viruses in vitro. To test this hypothesis, an Ad type 5-derived replication-deficient recombinant vector carrying the expression cassette for the cystic fibrosis gene (Ad.CFTR) was either incubated with A549 cells (a human-derived lung epithelial cell line) or instilled by bronchoscopic procedures into the airways of Rhesus monkeys. Ad.CFTR induced expression of ICAM-1 in A549 cells and up-regulated with time the basal levels of ICAM-1 mRNA in lung portions of Rhesus monkeys. These observations indicate that E1-E3-deleted replication-deficient adenoviral vectors are capable of inducing adhesion molecules known to play a role in inflammation.

Citations

May 26, 1999·American Journal of Respiratory Cell and Molecular Biology·D C Look, S L Brody
Jul 16, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H OgawaS Sone
Dec 14, 2011·PloS One·Marit A van MeegenJeffrey M Beekman
Mar 29, 2002·Reviews in Medical Virology·H Subak-SharpeJ D Pitts
Apr 27, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Dawn M FlahertyGary W Hunninghake
Dec 17, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Peng H TanAndrew J T George
Mar 25, 2005·American Journal of Physiology. Cell Physiology·Polly E MattilaBruce Walcheck
Oct 23, 2001·The Journal of Biological Chemistry·T D Joseph, D C Look
Dec 8, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hshi-Chi KooJeffrey A Kazzaz

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.