Icariin doped bioactive glasses seeded with rat adipose-derived stem cells to promote bone repair via enhanced osteogenic and angiogenic activities

Life Sciences
Xingzhi JingYaping Ye

Abstract

Cell communication between mesenchymal stem cells and blood vessel cells are crucial for bone repair. We have previously shown that the phyto-molecule icariin significantly promoted osteogenic differentiation of rat adipose-derived stem cells (ASCs). In the present study, we aimed to investigate the relationship between icariin induced osteogenic differentiation of ASCs and angiogenesis of rat endothelial progenitor cells (EPCs). Besides, we used icariin doped 45S5 Bioglass seeded with ASCs to promote bone healing in rat calvarial bone defect models. The conditioned medium from undifferentiated ASCs (ASCs-CM) and icariin induced ASCs (Icariin-ASCs-CM) was obtained and the vascular endothelial growth factor (VEGF) protein secretion level was measured. The angiogenic capacity and molecular mechanism of ASC-CM and Icariin-ASCs-CM on rat EPCs was analyzed. Rat calvarial bone defect models were established and treated with scaffolds implantation. Micro-CT imaging, histological and immunohistological staining were performed on the isolated specimens at 12 weeks post-surgery. VEGF protein expression was significantly increased after icariin treatment with the highest expression in the 10-7 M icariin group. Icariin-ASCs-CM obviously in...Continue Reading

Citations

Feb 27, 2020·Tissue Engineering. Part B, Reviews·Mirasbek KuterbekovCatherine Picart
Dec 19, 2019·Frontiers in Bioengineering and Biotechnology·Saeid KargozarRobert G Hill
Jun 17, 2020·Chemical Society Reviews·Saeid KargozarMasoud Mozafari
Jun 5, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Smriti GuptaSairam Krishnamurthy

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