Icosahedral bacteriophage ΦX174 forms a tail for DNA transport during infection

Nature
Lei SunBentley A Fane

Abstract

Prokaryotic viruses have evolved various mechanisms to transport their genomes across bacterial cell walls. Many bacteriophages use a tail to perform this function, whereas tail-less phages rely on host organelles. However, the tail-less, icosahedral, single-stranded DNA ΦX174-like coliphages do not fall into these well-defined infection processes. For these phages, DNA delivery requires a DNA pilot protein. Here we show that the ΦX174 pilot protein H oligomerizes to form a tube whose function is most probably to deliver the DNA genome across the host's periplasmic space to the cytoplasm. The 2.4 Å resolution crystal structure of the in vitro assembled H protein's central domain consists of a 170 Å-long α-helical barrel. The tube is constructed of ten α-helices with their amino termini arrayed in a right-handed super-helical coiled-coil and their carboxy termini arrayed in a left-handed super-helical coiled-coil. Genetic and biochemical studies demonstrate that the tube is essential for infectivity but does not affect in vivo virus assembly. Cryo-electron tomograms show that tubes span the periplasmic space and are present while the genome is being delivered into the host cell's cytoplasm. Both ends of the H protein contain tra...Continue Reading

References

May 24, 1991·Science·A LupasJ Stock
Jan 1, 1974·Journal of Virology·J M Bowes, C E Dowell
Oct 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·A B Burgess
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A AoyamaM Hayashi
Jan 1, 1996·Journal of Structural Biology·J R KremerJ R McIntosh
Jan 1, 1996·Methods in Enzymology·A Lupas
Mar 15, 2000·Journal of Molecular Biology·W P Russ, D M Engelman
Oct 9, 2001·Bioinformatics·G E Tusnády, I Simon
Dec 9, 2003·Trends in Biochemical Sciences·Markus Gruber, Andrei N Lupas
Apr 1, 1951·Proceedings of the National Academy of Sciences of the United States of America·L PAULINGH R BRANSON
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Jul 18, 2006·Journal of Molecular Biology·Ying A WangEdward H Egelman
Dec 14, 2006·Acta Crystallographica. Section D, Biological Crystallography·Airlie J McCoy
Nov 4, 2008·Journal of Bacteriology·Gerardo L PerezStanley Maloy
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Thomas J SilhavySuzanne Walker
Apr 19, 2011·Nature Structural & Molecular Biology·Adam S OliaGino Cingolani
Mar 27, 2012·Current Opinion in Structural Biology·Derek N WoolfsonAndrew R Thomson
May 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jun LiuWilliam Margolin
Feb 7, 2013·Nature Reviews. Microbiology·Ian J Molineux, Debabrata Panja
Oct 3, 2013·PLoS Biology·Bibiana PeraltaNicola G A Abrescia
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor
Jan 1, 1997·Methods in Enzymology·Sylvie Doublié
Jan 1, 1997·Methods in Enzymology·Liang Tong, Michael G Rossmann

❮ Previous
Next ❯

Citations

May 21, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yuriy ChabanElena V Orlova
Aug 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Bo HuJun Liu
May 31, 2015·Molecular Biology and Evolution·Sarah M Doore, Bentley A Fane
May 29, 2016·Environmental Microbiology·Karin HolmfeldtMatthew B Sullivan
Feb 14, 2016·Virology·Sarah M Doore, Bentley A Fane
Jan 27, 2015·Structure·E H EgelmanV P Conticello
Oct 25, 2014·Science·Andrew R ThomsonDerek N Woolfson
Oct 27, 2015·Chemical Society Reviews·Bas J G E PietersJasmin Mecinović
Apr 14, 2017·Journal of Virology·Jingwei Xu, Ye Xiang
Sep 7, 2014·Applied and Environmental Microbiology·Simon J LabrieSylvain Moineau
Sep 18, 2015·Microbiology and Molecular Biology Reviews : MMBR·Jan P Buchmann, Edward C Holmes
Feb 3, 2016·Journal of Bacteriology·Madeline M FarleyJun Liu
Oct 16, 2015·Nature Communications·Sudipta MondalEhud Gazit
Mar 7, 2014·Molecular Biology and Evolution·Sarah M DooreBentley A Fane
Dec 9, 2014·IUCrJ·Nuria VerdaguerMathur R N Murthy
Nov 14, 2019·Proceedings of the National Academy of Sciences of the United States of America·Paul R JaschkeDrew Endy
Feb 24, 2015·The Journal of Biological Chemistry·Verónica A González-GarcíaJosé L Carrascosa
Jul 8, 2020·Annual Review of Virology·Emmanuelle R J QueminKay Grünewald
Jul 1, 2016·Journal of Virology·Aaron P Roznowski, Bentley A Fane
Oct 10, 2018·Nature Communications·Guto G RhysDerek N Woolfson
Aug 7, 2017·Biophysical Reports·Jesús G Galaz-Montoya, Steven J Ludtke
Jan 11, 2019·Nature·Michaela J ConleyDavid Bhella
Dec 13, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yingyuan SunMichael G Rossmann
Feb 6, 2020·Nature Reviews. Microbiology·Moïra B DionSylvain Moineau
Jun 26, 2020·Viruses·Aaron P RoznowskiBentley A Fane
Jul 1, 2019·Current Opinion in Virology·Daniel J GoetschiusSusan Hafenstein
Feb 13, 2021·Peptide Science·Haley I MerrittParamjit S Arora
Jun 21, 2020·Virology·Dominic Y Logel, Paul R Jaschke
Jul 11, 2021·Proceedings of the National Academy of Sciences of the United States of America·Paul C KirchbergerHoward Ochman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Related Papers

Research in Microbiology
L LetellierP Boulanger
Proceedings of the National Academy of Sciences of the United States of America
Takashi FujiiKeiichi Namba
Current Topics in Microbiology and Immunology
Tobias J TuthillDavid J Rowlands
© 2021 Meta ULC. All rights reserved