ICP0 is not required for efficient stress-induced reactivation of herpes simplex virus type 1 from cultured quiescently infected neuronal cells

Journal of Virology
Craig S MillerR J Jacob

Abstract

Viral genes sufficient and required for herpes simplex virus type 1 (HSV-1) reactivation were identified using neuronally differentiated PC12 cells (ND-PC12 cells) in which quiescent infections with wild-type and recombinant strains were established. In this model, the expression of ICP0, VP16, and ICP4 from adenovirus vectors was sufficient to reactivate strains 17+ and KOS. The transactivators induced similar levels of reactivation with KOS; however, 17+ responded more efficiently to ICP0. To identify viral transactivators required for reactivation, we examined quiescently infected PC12 cell cultures (QIF-PC12 cell cultures) established with HSV-1 deletion mutants R7910 (deltaICP0), KD6 (deltaICP4), and in1814, a virus containing an insertion mutation in VP16. Although growth of these mutant viruses was impaired in ND-PC12 cells, R7910 and in1814 reactivated at levels equivalent to or better than their respective parental controls following stress (i.e., heat or forskolin) treatment. After treatment with trichostatin A, in1814 and 17+ reactivated efficiently, whereas the F strain and R7910 reactivated inefficiently. In contrast, KD6 failed to reactivate. In experiments with the recombinant KM100, which contains the in1814 mut...Continue Reading

References

Sep 1, 1976·Survey of Ophthalmology·J G Stevens
Jan 1, 1992·Journal of Virology·S P WeinheimerD R O'Boyle
May 1, 1992·Journal of Virology·P A JohnsonT Friedmann
Apr 1, 1991·The Journal of General Virology·R A Harris, C M Preston
Oct 1, 1988·The Journal of General Virology·C I AceC M Preston
May 1, 1985·Molecular and Cellular Biology·M P Quinlan, D M Knipe
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·I H Gelman, S Silverstein
Jun 1, 1972·The Journal of General Virology·T J HillA P Roome
Aug 27, 1971·Science·J G Stevens, M L Cook
Sep 1, 1994·The Journal of General Virology·T BlockN W Fraser
Apr 1, 1994·Journal of Virology·C A SmibertJ R Smiley

❮ Previous
Next ❯

Citations

Dec 7, 2011·Journal of Neurovirology·Nancy M SawtellRichard L Thompson
Apr 5, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ta-Chiang LiuRobert L Martuza
Sep 15, 2007·Journal of Virology·Tracy Terry-AllisonNeal A DeLuca
Jul 30, 2011·Future Virology·Christopher M TraylenShaw M Akula
Jan 29, 2013·Journal of Neurovirology·Ketna Volcy, Nigel W Fraser
Dec 14, 2011·FEMS Microbiology Reviews·Michael P NicollStacey Efstathiou
Jan 30, 2019·Viruses·Nicholas L BairdAbel Viejo-Borbolla

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.