ICSI outcome in patients with high DNA fragmentation: Testicular versus ejaculated spermatozoa

Andrologia
M ArafaH Elbardisi

Abstract

Sperm DNA fragmentation (SDF) has emerged as an important biomarker in the assessment of male fertility potential with contradictory results regarding its effect on ICSI. The aim of this study was to evaluate intracytoplasmic sperm injection (ICSI) outcomes in male patients with high SDF using testicular versus ejaculated spermatozoa. This is a prospective study on 36 men with high-SDF levels who had a previous ICSI cycle from their ejaculates. A subsequent ICSI cycle was performed using spermatozoa retrieved through testicular sperm aspiration. Results of the prior ejaculate ICSI were compared with those of the TESA-ICSI. The mean (SD) SDF level was 56.36% (15.3%). Overall, there was no difference in the fertilization rate and embryo grading using ejaculate and testicular spermatozoa (46.4% vs. 47.8%, 50.2% vs. 53.4% respectively). However, clinical pregnancy was significantly higher in TESA group compared to ejaculated group (38.89% [14 of 36] vs. 13.8% [five of 36]). Moreover, 17 live births were documented in TESA group, and only three live births were documented in ejaculate group (p < .0001). We concluded that the use of testicular spermatozoa for ICSI significantly increases clinical pregnancy rate as well as live-birth ...Continue Reading

References

May 2, 2003·Human Reproduction·Mehdi BenchaibJean François Guérin
Oct 11, 2005·Fertility and Sterility·José Luis FernándezChristopher De Jonge
Jan 10, 2006·Journal of Andrology·Kazim R ChohanDouglas T Carrell
Oct 25, 2011·Reproductive Biomedicine Online·Marga EsbertGloria Calderón
Dec 4, 2012·Reproductive Biomedicine Online·L SimonS E M Lewis

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Citations

Apr 17, 2019·Panminerva Medica·Leonardo S Lopes, Sandro C Esteves
May 2, 2018·Arab Journal of Urology·Chak-Lam Cho, Ashok Agarwal
Aug 7, 2019·The World Journal of Men's Health·Ashok AgarwalRalf Henkel
Jul 30, 2018·Journal of Assisted Reproduction and Genetics·C L O'NeillG D Palermo
Dec 12, 2019·F1000Research·Sandro C Esteves, Matheus Roque

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