Id2 is a primary partner for the E2-2 basic helix-loop-helix transcription factor in the human placenta

Molecular and Cellular Endocrinology
Yi-Ping LiuThaddeus G Golos

Abstract

We screened a term placental cDNA library by the yeast two-hybrid approach with Id2, a negative regulator of basic helix-loop-helix (bHLH) factors. Of the clones obtained, approximately one-third were the E2-2 bHLH transcription factor. Id2 and E2-2 were shown to interact in direct two-hybrid assays in yeast cells, as well as immunoprecipitation assays in mammalian cells. Immunohistochemical analysis demonstrated co-localization of both Id2 and E2-2 in placental trophoblasts. Co-transfection of JEG-3 cells with E2-2 and Id2, and a luciferase reporter construct under the control of the human chorionic gonadotropin alpha-subunit promoter revealed that E2-2 had a negative effect on CGalpha-subunit transcription, which could be relieved by overexpression of Id2. The library was in turn rescreened with E2-2, and Id2 and Id1 were essentially the only clones obtained. We conclude that Id2 is a primary binding partner for the bHLH transcription factor E2-2 in the human placenta.

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Citations

Nov 25, 2005·Stem Cells and Development·Kaisa L SelesniemiThomas L Brown
Jan 26, 2006·Stem Cells and Development·Kaisa SelesniemiThomas L Brown
Jun 10, 2011·Development·Aurélie J KrolOlivier Pourquié
Apr 28, 2010·Molecular Reproduction and Development·Yufen XieDan A Rappolee
Aug 16, 2014·Immunological Reviews·Haiyan S Li, Stephanie S Watowich
May 12, 2016·Stem Cells and Development·Kaisa SelesniemiThomas L Brown
Aug 18, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Verónica M Negrón-PérezPeter J Hansen
Jan 5, 2007·Journal of the American Society of Nephrology : JASN·Yingjian LiYouhua Liu
Jan 21, 2006·Molecular Reproduction and Development·Terla MuirMichael K Skinner

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